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Study of Phylogenetic Relationship among Fusarium oxysporum f. sp. dianthi Isolates and Development of a Practical Tool for Simple Population Analyses

AutorCañizares, M. Carmen; Gómez-Lama Cabanás, Carmen ; García-Pedrajas, María D.; Pérez-Artés, Encarnación
Fecha de publicaciónoct-2014
CitaciónXVII Congreso de la Sociedad Española de Fitopatología (2014)
ResumenFusarium wilt, caused by Fusarium oxysporum f. sp. dianthi (Fod), is the most important carnation disease worldwide. Sequence analyses of partial β-tubuline (β-tub) and translation elongation factor 1α (EF-1α) genes, and the full-length ribosomal DNA intergenic spacer (IGS) were conducted to resolve phylogenetic relationships in a collection of Fod isolates obtained from the most important growing areas in Spain during the period 1998–2011, along with some representatives from Italy. We found that among the three different gene regions under study, the IGS sequence was the best choice to resolve phylogenetic relationships among Fod isolates. Thus, the phylogenetic tree generated with the complete IGS region was the only one showing a perfect clustering of isolates of the same molecular group (virulence grouping) and vegetative compatibility group (VCG). In order to develop a more practical tool, based on a shorter DNA sequence, to quickly analyze population structure of Fod isolates, we examined in detail IGS nucleotide alignments and identified a region of approximately 300pb that accumulates enough “informative” changes to resolve intraspecific relationships in Fod. Moreover, the “condensed” alignment of this short IGS region showing only the informative positions, revealed the existence of virulence group-discriminating positions. Thus, in this study, in addition to clarifying the phylogenetic relationships among Fod isolates of the recently described race-groups by using multi-gene genealogies, we have developed simple tools for the phylogenetic analyses of Fod population and the determination of the molecular group (level of aggressiveness) of new Fod isolates.
DescripciónTrabajo presentado en el XVII Congreso de la Sociedad Española de Fitopatología, celebrado en Lleida del 7 al 10 de octubre de 2014.
URIhttp://hdl.handle.net/10261/159319
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