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Integrative taxonomy of the stunt nematodes of the genera Bitylenchus and Tylenchorhynchus (Nematoda, Telotylenchidae) with description of two new species and a molecular phylogeny

AuthorsHandoo, Zafar A.; Palomares Rius, Juan E. ; Cantalapiedra-Navarrete, C. ; Liébanas, Gracia; Subbotin, Sergei A.; Castillo, Pablo
Bayesian inference
Cryptic species
D2–D3 expansion segments of the large ribosomal subunit (28S)
Internal transcribed spacer
Shimodaira–Hasegawa test
Stylet knob inclination
Tail tip annulation
Issue DateOct-2014
PublisherJohn Wiley & Sons
CitationZoological Journal of the Linnean Society 172(2): 231-264 (2014)
AbstractStunt nematodes are characterized by phenotypic plasticity, with overlapping morphology and morphometry leading to potential misidentification. Consequently, the application of integrative taxonomic approaches is useful to species delimitation based on a combination of different perspectives, e.g. morphology and DNA sequences. We conducted nematode surveys in cultivated and natural environments in Spain and the USA, from which we identified 18 known species of the family Telotylenchidae and two new taxa within the studied samples. These species were morphologically, morphometrically, and molecularly characterized. The results of light and scanning electron microscopic observations, and molecular and phylogenetic analysis also allowed two new species to be distinguished, described herein as Bitylenchus hispaniensis sp. nov. and Tylenchorhynchus mediterraneus sp. nov. The phylogenetic analysis was carried out using molecular data from nuclear ribosomal DNA genes [D2–D3 expansion segments of the large ribosomal subunit (28S), internal transcribed spacer (ITS), and partial small ribosomal subunit (18S)]. We also provide here a test of alternative hypotheses that confirms the monophyly of both Tylenchorhynchus and Bitylenchus sensu Siddiqi's classification but does not support Fortuner & Luc's conceptual view of Tylenchorhynchus as a large genus. Ancestral state reconstructions of several diagnostic morphological characters using a maximum parsimony approach showed congruence in morphological and molecular evolution for stylet knob inclination and tail tip annulation. Our analysis emphasizes some of the problems related to the taxonomy and phylogeny of nematodes of Telotylenchinae. © 2014 The Linnean Society of London
Publisher version (URL)http://doi.org/10.1111/zoj.12175
Appears in Collections:(IAS) Artículos
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