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Title

Stem cell-based gene delivery mediated by cationic niosomes for bone regeneration

AuthorsAttia, Noah; Mashal, Mohamed; Grijalvo, Santiago CSIC ORCID; Eritja Casadellà, Ramón CSIC ORCID ; Zárate, Jon; Puras, Gustavo; Pedraz, Jose Luis
KeywordsBone regeneration
Gene delivery
Niosomes
Stem cells
Issue Date21-Nov-2017
PublisherElsevier
CitationNanomedicine - Nanotechnology Biology and Medicine: 14 (2):521-531 (2017)
AbstractBone morphogenetic protein-7(BMP-7) plays a pivotal role in the transformation of mesenchymal stem cells (MSCs) into bone. However, its impact is hampered due to its short half-life. Therefore, gene therapy may be an interesting approach to deliver BMP-7 gene to D1-MSCs. In this manuscript we prepared and characterized niosomes based on cationic lipid 2,3-di(tetradecyloxy)propan-1-amine, combined with polysorbate 80 for gene delivery purposes. Niosomes were characterized and combined initially with pCMS-EGFP reporter plasmid, and later with pUNO1-hBMP-7 plasmid to evaluate osteogenesis differentiation. Additionally, specific blockers of most relevant endocytic pathways were used to evaluate the intracellular disposition of complexes. MSCs transfected with niosomes showed increased growth rate, enhanced alkaline phosphatase activity (ALP) and extracellular matrix deposition which suggested the formation of osteoblast-like cells. We concluded that hBMP-7-transfected MSCs could be considered not only as an effective delivery tool of hBMP-7, but also as proliferating and bone forming cells for bone regeneration.
Publisher version (URL)10.1016/j.nano.2017.11.005
URIhttp://hdl.handle.net/10261/159063
DOIhttp://dx.doi.org/10.1016/j.nano.2017.11.005
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