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Título

Autophagy is activated and involved in cell death with participation of cathepsins during stress-induced microspore embryogenesis in barley

AutorBárány, Ivett ; Berenguer, Eduardo; Solís, María Teresa ; Pérez-Pérez, Yolanda; Santamaría, María Estrella; Crespo, José L. ; Risueño, María Carmen ; Díaz, Isabel; Testillano, P.S.
Palabras claveAutophagy
Barley
Caspase-like activity
Bathepsins
Cell death
Cysteine C1A proteases
Microspore embryogenesis
ROS
Stress response.
Fecha de publicación4-ene-2018
EditorSociety for Experimental Biology
Oxford University Press
CitaciónJ. Exp. Bot. erx455 (2018)
ResumenMicrospores are reprogrammed towards embryogenesis by stress. Many microspores die after this stress, limiting the efficiency of microspore embryogenesis. Autophagy is a degradation pathway that plays critical roles in stress response and cell death. In animals, cathepsins have an integral role in autophagy by degrading autophagic material; less is known in plants. Plant cathepsins are papain-like C1A cysteine proteases involved in many physiological processes, including programmed cell death. We have analysed the involvement of autophagy in cell death, in relation to cathepsin activation, during stress-induced microspore embryogenesis in Hordeum vulgare. After stress, reactive oxygen species (ROS) and cell death increased and autophagy was activated, including HvATG5 and HvATG6 up-regulation and increase of ATG5, ATG8, and autophagosomes. Concomitantly, cathepsin L/F-, B-, and H-like activities were induced, cathepsin-like genes HvPap-1 and HvPap-6 were up-regulated, and HvPap-1, HvPap-6, and HvPap-19 proteins increased and localized in the cytoplasm, resembling autophagy structures. Inhibitors of autophagy and cysteine proteases reduced cell death and promoted embryogenesis. The findings reveal a role for autophagy in stress-induced cell death during microspore embryogenesis, and the participation of cathepsins. Similar patterns of activation, expression, and localization suggest a possible connection between cathepsins and autophagy. The results open up new possibilities to enhance microspore embryogenesis efficiency with autophagy and/or cysteine protease modulators.
Descripción16 p.-8 fig.-1 tab.-1 tab. supl.- 1 mat. supl.
Versión del editorhttps://academic.oup.com/jxb/advance-article-abstract/doi/10.1093/jxb/erx455/4791010
URIhttp://hdl.handle.net/10261/158937
DOI10.1093/jxb/erx455
E-ISSN1460-2431
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