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Título

Insights into the inhibited form of the redox-sensitive SufE-like sulfur acceptor CsdE

AutorPeña-Soler, Esther CSIC; Aranda, Juan; López-Estepa, Miguel CSIC; Gómez, Sara; Garces, Fernando CSIC; Coll, Miquel CSIC ORCID ; Fernández, Francisco J. CSIC ORCID; Tuñon, Iñaki; Vega, María Cristina CSIC ORCID
Fecha de publicación18-oct-2017
EditorPublic Library of Science
CitaciónPLoS ONE 12(10): e0186286 (2017)
ResumenSulfur trafficking in living organisms relies on transpersulfuration reactions consisting in the enzyme-catalyzed transfer of S atoms via activated persulfidic S across protein-protein interfaces. The recent elucidation of the mechanistic basis for transpersulfuration in the CsdA-CsdE model system has paved the way for a better understanding of its role under oxidative stress. Herein we present the crystal structure of the oxidized, inactivated CsdE dimer at 2.4 Å resolution. The structure sheds light into the activation of the Cys61 nucleophile on its way from a solvent-secluded position in free CsdE to a fully extended conformation in the persulfurated CsdA-CsdE complex. Molecular dynamics simulations of available CsdE structures allow to delineate the sequence of conformational changes underwent by CsdE and to pinpoint the key role played by the deprotonation of the Cys61 thiol. The low-energy subunit orientation in the disulfide-bridged CsdE dimer demonstrates the likely physiologic relevance of this oxidative dead-end form of CsdE, suggesting that CsdE could act as a redox sensor in vivo.
Descripción17 p.-8 fig.
Versión del editorhttps://doi.org/10.1371/journal.pone.0186286
URIhttp://hdl.handle.net/10261/158683
DOI10.1371/journal.pone.0186286
E-ISSN1932-6203
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