English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/158447
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Polysaccharides as antiviral agents: antiviral activity of carrageenan

AuthorsGonzález, M. E; Alarcón, Balbino ; Carrasco, Luís
Issue Date1987
PublisherAmerican Society for Microbiology
CitationAntimicrobial Agents and Chemotherapy 31: 1388- 1393 (1987)
AbstractA number of polysaccharides showed good antiviral activity against several animal viruses. At 5 μg/ml, carrageenan prevented the cell monolayer from destruction by herpes simplex virus type 1 (HSV-1) growth. At 10 μg/ml, carrageenan reduced the formation of new infectious HSV-1 by almost five logs. No cytotoxic effects were detected with concentrations of carrageenan up to 200 μg/ml. When 10 μg of carrageenan per ml was added at the beginning of HSV-1 infection of HeLa cells, there was potent inhibition of viral protein synthesis, and the cells continued synthesizing cellular proteins. This did not occur if carrageenan was added 1 h after HSV-1 infection. The use of [S]methionine-labeled virions to analyze the entry of HSV-1 or Semliki Forest virions into cells indicated that carrageenan and no effect on virus attachment or virus entry. Moreover, carrageenan did not block the early permeabilization of cells to the toxic protein alpha-sarcin. These results suggest that this sulfated polysaccharide inhibits a step in virus replication subsequent to viral internalization but prior to the onset of late viral protein synthesis.
URIhttp://hdl.handle.net/10261/158447
Identifiersissn: 0066-4804
Appears in Collections:(CBM) Artículos
Files in This Item:
File Description SizeFormat 
1987 Antimicrob Agents Chem Carrag.pdf1,42 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.