Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/158427
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Large-Scale Transcriptome Analysis in Faba Bean (Vicia faba L.) under Ascochyta fabae Infection |
Autor: | Ocaña-Moral, Sara; Seoane, Pedro; Bautista, Rocío; Palomino, Carmen CSIC; Claros, Gonzalo M.; Torres, Ana M.; Madrid, Eva CSIC ORCID | Fecha de publicación: | 12-ago-2015 | Editor: | Public Library of Science | Citación: | PLoS ONE 10(8): e0135143 (2015) | Resumen: | Faba bean is an important food crop worldwide. However, progress in faba bean genomics lags far behind that of model systems due to limited availability of genetic and genomic information. Using the Illumina platform the faba bean transcriptome from leaves of two lines (29H and Vf136) subjected to Ascochyta fabae infection have been characterized. De novo transcriptome assembly provided a total of 39,185 different transcripts that were functionally annotated, and among these, 13,266 were assigned to gene ontology against Arabidopsis. Quality of the assembly was validated by RT-qPCR amplification of selected transcripts differentially expressed. Comparison of faba bean transcripts with those of better-characterized plant genomes such as Arabidopsis thaliana, Medicago truncatula and Cicer arietinum revealed a sequence similarity of 68.3%, 72.8% and 81.27%, respectively. Moreover, 39,060 single nucleotide polymorphism (SNP) and 3,669 InDels were identified for genotyping applications. Mapping of the sequence reads generated onto the assembled transcripts showed that 393 and 457 transcripts were overexpressed in the resistant (29H) and susceptible genotype (Vf136), respectively. Transcripts involved in plant-pathogen interactions such as leucine rich proteins (LRR) or plant growth regulators involved in plant adaptation to abiotic and biotic stresses were found to be differently expressed in the resistant line. The results reported here represent the most comprehensive transcript database developed so far in faba bean, providing valuable information that could be used to gain insight into the pathways involved in the resistance mechanism against A. fabae and to identify potential resistance genes to be further used in marker assisted selection. | Versión del editor: | http://doi.org/10.1371/journal.pone.0135143 | URI: | http://hdl.handle.net/10261/158427 | DOI: | 10.1371/journal.pone.0135143 | ISSN: | 1932-6203 |
Aparece en las colecciones: | (IAS) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
ascochyta_fabae_Ocana.pdf | 1,07 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
22
checked on 16-mar-2024
SCOPUSTM
Citations
40
checked on 14-mar-2024
WEB OF SCIENCETM
Citations
36
checked on 26-feb-2024
Page view(s)
317
checked on 19-mar-2024
Download(s)
198
checked on 19-mar-2024