English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/158255
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Copper and ectopic expression of the Arabidopsis transport protein COPT1 alter iron homeostasis in rice (Oryza sativa L.)

AutorAndrés Bordería, Amparo; Andrés, Fernando; Garcia Molina, Antoni; Perea García, Ana ; Domingo, Concha; Puig, Sergi ; Peñarrubia, Lola
Palabras claveCopper
Iron
Metal transport
Oryza sativa
COPT1
OsIRO2
Fecha de publicación19-jun-2017
EditorSpringer
CitaciónPlant Molecular Biology 95 (1-2): 17-32 (2017)
ResumenHigher plants have developed sophisticated mechanisms to efficiently acquire and use micronutrients such as copper and iron. However, the molecular mechanisms underlying the interaction between both metals remain poorly understood. In the present work, we study the effects produced on iron homeostasis by a wide range of copper concentrations in the growth media and by altered copper transport in Oryza sativa plants. Gene expression profiles in rice seedlings grown under copper excess show an altered expression of genes involved in iron homeostasis compared to standard control conditions. Thus, ferritin OsFER2 and ferredoxin OsFd1 mRNAs are down-regulated whereas the transcriptional iron regulator OsIRO2 and the nicotianamine synthase OsNAS2 mRNAs rise under copper excess. As expected, the expression of OsCOPT1, which encodes a high-affinity copper transport protein, as well as other copper-deficiency markers are down-regulated by copper. Furthermore, we show that Arabidopsis COPT1 overexpression (C1OE) in rice causes root shortening in high copper conditions and under iron deficiency. C1OE rice plants modify the expression of the putative iron-sensing factors OsHRZ1 and OsHRZ2 and enhance the expression of OsIRO2 under copper excess, which suggests a role of copper transport in iron signaling. Importantly, the C1OE rice plants grown on soil contain higher endogenous iron concentration than wild-type plants in both brown and white grains. Collectively, these results highlight the effects of rice copper status on iron homeostasis, which should be considered to obtain crops with optimized nutrient concentrations in edible parts.
Versión del editorhttps://doi.org/10.1007/s11103-017-0622-8
URIhttp://hdl.handle.net/10261/158255
DOI10.1007/s11103-017-0622-8
ISSN0167-4412
E-ISSN1573-5028
Aparece en las colecciones: (IATA) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
PMB-Andres-2017.pdfArtículo principal1,59 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.