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Title

Regulation of phospholipid hydrolysis and signaling by cPLA2, iPLA2 and sPLA2 in activated macrophages

AuthorsBalsinde, Jesús
Issue Date2015
Citation6th International Conference on Phospholipase A2 and Lipid Mediators (2015)
AbstractPhospholipase A2s generate lipid mediators that constitute an important component of the integrated response of macrophages to stimuli of the innate immune response. Because these cells contain multiple phospholipase A2 forms, the challenge is to elucidate the roles that each of these forms plays in regulating normal cellular processes and in disease pathogenesis. We have applied mass spectrometry¿based lipid profiling to study the substrate specificities of various PLA2s during inflammatory activation of macrophages with zymosan.1-3 Using selective inhibitors, we find that, contrary to cPLA2¿, iPLA2¿ spares arachidonate-containing phospholipids and hydrolyzes only those that do not contain arachidonate. Analyses of the lysophospholipids generated during activation reveal that one of the major species produced, palmitoyl-glycerophosphocholine, is generated by iPLA2¿, with minimal or no involvement of cPLA2¿. The other major species produced, stearoyl- glycerophosphocholine, is generated primarily by cPLA2¿.4 Studies regarding the expression and biological role of group V sPLA2 during macrophage polarizaton will also be discussed.
DescriptionTranscription of the lecture presented on Tuesday, February 10, 2015 at the 6th International Conference on Phospholipase A2 and Lipid Mediators: from bench to translational medicine, Tokyo, Japan.
URIhttp://hdl.handle.net/10261/158167
Appears in Collections:(IBGM) Comunicaciones congresos
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