English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/158141
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Endoplasmic reticulum stress sensor IRE1α enhances IL-23 expression by human dendritic cells

AuthorsMárquez, Saioa; Herrero, Carmen ; Alonso, Sara ; Azogil, Alicia; Montero, Olimpio; Fernández, Nieves ; Sánchez Crespo, Mariano
Issue Date2017
PublisherFrontiers Media
CitationFrontiers in Immunology 8: 639 (2017)
AbstractHuman monocyte-derived dendritic cells (DCs) exposed to pathogen-associated molecular patterns (PAMPs) undergo bioenergetic changes that influence the immune response. We found that stimulation with PAMPs enhanced glycolysis in DCs, whereas oxidative phosphorylation remained unaltered. Glucose starvation and the hexokinase inhibitor 2-deoxy-d-glucose (2-DG) modulated cytokine expression in stimulated DCs. Strikingly, IL23A was markedly induced upon 2-DG treatment, but not during glucose deprivation. Since 2-DG can also rapidly inhibit protein N-glycosylation, we postulated that this compound could induce IL-23 in DCs via activation of the endoplasmic reticulum (ER) stress response. Indeed, stimulation of DCs with PAMPs in the presence of 2-DG robustly activated inositol-requiring protein 1a (IRE1α) signaling and to a lesser extent the PERK arm of the unfolded protein response. Additional ER stressors such as tunicamycin and thapsigargin also promoted IL-23 expression by PAMP-stimulated DCs. Pharmacological, biochemical, and genetic analyses using conditional knockout mice revealed that IL-23 induction in ER stressed DCs stimulated with PAMPs was IRE1α/X-box binding protein 1-dependent upon zymosan stimulation. Interestingly, we further evidenced PERK-mediated and CAAT/enhancer-binding protein β-dependent trans-activation of IL23A upon lipopolysaccharide treatment. Our findings uncover that the ER stress response can potently modulate cytokine expression in PAMP-stimulated human DCs.
DescriptionThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).-- et al.
Publisher version (URL)https://doi.org/10.3389/fimmu.2017.00639
Identifiersdoi: 10.3389/fimmu.2017.00639
e-issn: 1664-3224
Appears in Collections:(IBGM) Artículos
Files in This Item:
File Description SizeFormat 
IRE1IL-23.pdf5,52 MBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.