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dc.contributor.authorHidalgo, Jorge-
dc.contributor.authorTeuber, Stefanie-
dc.contributor.authorMorera, Francisco J.-
dc.contributor.authorOjeda, Camila-
dc.contributor.authorFlores, Carlos A.-
dc.contributor.authorHidalgo, María A.-
dc.contributor.authorNúñez, Lucía-
dc.contributor.authorVillalobos, Carlos-
dc.contributor.authorBurgos, Rafael A.-
dc.date.accessioned2017-12-13T08:31:09Z-
dc.date.available2017-12-13T08:31:09Z-
dc.date.issued2017-
dc.identifierdoi: 10.3390/ijms18040750-
dc.identifiere-issn: 1422-0067-
dc.identifierissn: 1661-6596-
dc.identifier.citationInternational Journal of Molecular Sciences 18(4): 750 (2017)-
dc.identifier.urihttp://hdl.handle.net/10261/158128-
dc.descriptionThis article belongs to the Special Issue Anthocyanins.-
dc.description.abstractAnthocyanins are pigments with antihyperglycemic properties, and they are potential candidates for developing functional foods for the therapy or prevention of Diabetes mellitus type 2 (DM2). The mechanism of these beneficial effects of anthocyanins are, however, hard to explain, given their very low bioavailability due to poor intestinal absorption.We propose that free fatty acid receptor 1 (FFA1, also named GPR40), is involved in an inhibitory effect of the anthocyanidin delphinidin over intestinal glucose absorption.We show the direct effects of delphinidin on the intestine using jejunum samples from RF/J mice, and the human intestinal cell lines HT-29, Caco-2, and NCM460. By the use of specific pharmacological antagonists, we determined that delphinidin inhibits glucose absorption in both mouse jejunum and a human enterocytic cell line in a FFA1-dependent manner. Delphinidin also affects the function of sodium-glucose cotransporter 1 (SGLT1). Intracellular signaling after FFA1 activation involved cAMP increase and cytosolic Ca oscillations originated from intracellular Ca stores and were followed by store-operated Ca entry. Taken together, our results suggest a new GPR-40 mediated local mechanism of action for delphinidin over intestinal cells that may in part explain its antidiabetic effect. These findings are promising for the search for new prevention and pharmacological treatment strategies for DM2 management.-
dc.description.sponsorshipThis work was supported by the Grant INNOVA CORFO 12IDL2-16254 and the CONICYT doctoral scholarship 21130644 (to Jorge Hidalgo) and the Grant BFU2015-70131-R from the Government of Spain.-
dc.description.sponsorshipWe acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI).-
dc.publisherMolecular Diversity Preservation International-
dc.relationMINECO/ICTI2013-2016/BFU2015-70131-R-
dc.relation.isversionofPublisher's version-
dc.rightsopenAccess-
dc.subjectSGLT1-
dc.subjectGlucose-
dc.subjectGPR40-
dc.subjectFFA1-
dc.subjectAnthocyanins-
dc.subjectDelphinidin-
dc.titleDelphinidin reduces glucose uptake in mice jejunal tissue and human intestinal cells lines through FFA1/GPR40-
dc.typeartículo-
dc.identifier.doi10.3390/ijms18040750-
dc.relation.publisherversionhttps://doi.org/10.3390/ijms18040750-
dc.date.updated2017-12-13T08:31:10Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.rights.licensehttps://creativecommons.org/licenses/by/4.0/-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.contributor.funderComisión Nacional de Investigación Científica y Tecnológica (Chile)-
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100002848es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003339es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
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