English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/158081
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Molecular determinants of Kv1.3 potassium channels-induced proliferation

AutorJiménez-Pérez, Laura; Cidad, Pilar ; Álvarez-Miguel, Inés; Santos-Hipólito, Alba; Torres-Merino, Rebeca; Alonso, Esperanza ; Fuente, Miguel A. de la; López-López, José R. ; Pérez-García, M. Teresa
Fecha de publicación2016
EditorAmerican Society for Biochemistry and Molecular Biology
CitaciónJournal of Biological Chemistry 291(7): 3569-3580 (2016)
ResumenChanges in voltage-dependent potassium channels (Kv channels) associate to proliferation in many cell types, including transfected HEK293 cells. In this system Kv1.5 overexpression decreases proliferation, whereas Kv1.3 expression increases it independently of KK fluxes. To identify Kv1.3 domains involved in a proliferation-associated signaling mechanism(s), we constructed chimeric Kv1.3-Kv1.5 channels and point-mutant Kv1.3 channels, which were expressed as GFP- or cherry-fusion proteins. We studied their trafficking and functional expression, combining immunocytochemical and electrophysiological methods, and their impact on cell proliferation. We found that the C terminus is necessary for Kv1.3-induced proliferation.We distinguished two residues (Tyr-447 and Ser-459) whose mutation to alanine abolished proliferation. The insertion into Kv1.5 of a sequence comprising these two residues increased proliferation rate. Moreover, Kv1.3 voltage-dependent transitions from closed to open conformation induced MEK-ERK1/2-dependent Tyr-447 phosphorylation. We conclude that the mechanisms for Kv1.3-induced proliferation involve the accessibility of key docking sites at the C terminus. For one of these sites (Tyr-447) we demonstrated the contribution of MEK/ERK-dependent phosphorylation, which is regulated by voltage-induced conformational changes.
Versión del editorhttps://doi.org/10.1074/jbc.M115.678995
URIhttp://hdl.handle.net/10261/158081
DOI10.1074/jbc.M115.678995
Identificadoresdoi: 10.1074/jbc.M115.678995
e-issn: 1083-351X
issn: 0021-9258
Aparece en las colecciones: (IBGM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
ChannelsProliferation.pdf2,59 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.