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Novel strains of Streptomyces coelicolor lacking two component systems involved in antibiotic production

AuthorsMinatelli, Sabrina; Antoraz, Sergio; Sevillano, Laura ; Santamaría, Ramón I. ; Díaz, Margarita
Issue Date2016
Citation13th Horizons in Molecular Biology (2016)
AbstractThe two-component systems (TCS) are important global pathways of regulation in bacteria. Our group study the TCSs involved in antibiotic regulation in Streptomyces. In previous work we have shown the great potential of Streptomyces coelicolor strains altered in TCSs to express different clusters from other organisms that present low antibiotic production. In this work we have generated new strains of S. coelicolor in which the TCS AbrB have been removed. The AbrB is a negative regulator of antibiotic production and morphological differentiation. Moreover, we have generated a second elimination of AbrA TCS from the mutant AbrB, getting a double mutant strain. The AbrA is also a negative regulator previously studied in our group. Both mutants were obtained by the REDIRECT procedure. The comparative analysis of mutants with the wild type strain was carried out in different solid and liquid mediums. The phenotype of both mutants was highly medium-dependent. We have found, however, that the double elimination of two negative TCSs regulators (AbrA and AbrB) is beneficial to increase the production of endogenous antibiotics in some culture medium such as LB or YEPD. However, in many of the conditions tested, the production was similar to that obtained with the single mutants. We believe that in the absence of AbrA and AbrB systems, others TCSs could be playing an important role in the regulation of the observed responses in these strains. The usefulness of these new strains of S. coelicolor in the heterologous expression of antibiotic routes has been initially checked with the Tetracenomycin C pathway, an aromatic polyketide produced by S. glaucescens. We have observed a high increase in Tetracenomycin production in the mutants strains compared with the wild type.
DescriptionResumen del trabajo presentado al 13th Horizons in Molecular Biology Congress, celebrado en Göttingen (Alemania) del 12 al 15 de septiembre de 2016.
Appears in Collections:(IBFG) Comunicaciones congresos
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