Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/157863
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Stein, Benjamin | - |
dc.contributor.author | Alonso, María Teresa | - |
dc.contributor.author | Zufall, Frank | - |
dc.contributor.author | Leinders-Zufall, Trese | - |
dc.contributor.author | Chamero, Pablo | - |
dc.date.accessioned | 2017-11-29T13:21:10Z | - |
dc.date.available | 2017-11-29T13:21:10Z | - |
dc.date.issued | 2016 | - |
dc.identifier | doi: 10.1371/journal.pone.0156092 | - |
dc.identifier | e-issn: 1932-6203 | - |
dc.identifier.citation | PLoS ONE 11(5): e0156092 (2016) | - |
dc.identifier.uri | http://hdl.handle.net/10261/157863 | - |
dc.description.abstract | In mice, social behaviors such as mating and aggression are mediated by pheromones and related chemosignals. The vomeronasal organ (VNO) detects olfactory information from other individuals by sensory neurons tuned to respond to specific chemical cues. Receptors expressed by vomeronasal neurons are implicated in selective detection of these cues. Nearly 400 receptor genes have been identified in the mouse VNO, but the tuning properties of individual receptors remain poorly understood, in part due to the lack of a robust heterologous expression system. Here we develop a herpes virus-based amplicon delivery system to overexpress three types of vomeronasal receptor genes and to characterize cell responses to their proposed ligands. Through Ca imaging in native VNO cells we show that virus-induced overexpression of V1rj2, V2r1b or Fpr3 caused a pronounced increase of responsivity to sulfated steroids, MHC-binding peptide or the synthetic hexapeptide W-peptide, respectively. Other related ligands were not recognized by infected individual neurons, indicating a high degree of selectivity by the overexpressed receptor. Removal of G-protein signaling eliminates Ca responses, indicating that the endogenous second messenger system is essential for observing receptor activation. Our results provide a novel expression system for vomeronasal receptors that should be useful for understanding the molecular logic of VNO ligand detection. Functional expression of vomeronasal receptors and their deorphanization provides an essential requirement for deciphering the neural mechanisms controlling behavior. | - |
dc.description.sponsorship | This work was supported by Deutsche Forschungsgemeinschaft grants CH 920/2-1 (PC) and Sonderforschungsbereich 894 projects A16 (TL-Z) and A17 (FZ), a University of Saarland HOMFORexzellent grant (PC), the Volkswagen Foundation (TL-Z). | - |
dc.publisher | Public Library of Science | - |
dc.relation.isversionof | Publisher's version | - |
dc.rights | openAccess | - |
dc.title | Functional overexpression of vomeronasal receptors using a herpes simplex virus type 1 (HSV-1)-derived amplicon | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1371/journal.pone.0156092 | - |
dc.relation.publisherversion | https://doi.org/10.1371/journal.pone.0156092 | - |
dc.date.updated | 2017-11-29T13:21:11Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.rights.license | http://creativecommons.org/licenses/by/4.0/ | - |
dc.contributor.funder | Saarland University | - |
dc.contributor.funder | German Research Foundation | - |
dc.contributor.funder | Volkswagen Foundation | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100001659 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100005690 | es_ES |
dc.identifier.pmid | 27195771 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
Aparece en las colecciones: | (IBGM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
HSV-1derived.PDF | 1,95 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
5
checked on 27-mar-2024
SCOPUSTM
Citations
9
checked on 23-abr-2024
WEB OF SCIENCETM
Citations
8
checked on 26-feb-2024
Page view(s)
239
checked on 23-abr-2024
Download(s)
372
checked on 23-abr-2024