English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/157863
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Functional overexpression of vomeronasal receptors using a herpes simplex virus type 1 (HSV-1)-derived amplicon

AuthorsStein, Benjamin; Alonso, María Teresa ; Zufall, Frank; Leinders-Zufall, Trese; Chamero, Pablo
Issue Date2016
PublisherPublic Library of Science
CitationPLoS ONE 11(5): e0156092 (2016)
AbstractIn mice, social behaviors such as mating and aggression are mediated by pheromones and related chemosignals. The vomeronasal organ (VNO) detects olfactory information from other individuals by sensory neurons tuned to respond to specific chemical cues. Receptors expressed by vomeronasal neurons are implicated in selective detection of these cues. Nearly 400 receptor genes have been identified in the mouse VNO, but the tuning properties of individual receptors remain poorly understood, in part due to the lack of a robust heterologous expression system. Here we develop a herpes virus-based amplicon delivery system to overexpress three types of vomeronasal receptor genes and to characterize cell responses to their proposed ligands. Through Ca imaging in native VNO cells we show that virus-induced overexpression of V1rj2, V2r1b or Fpr3 caused a pronounced increase of responsivity to sulfated steroids, MHC-binding peptide or the synthetic hexapeptide W-peptide, respectively. Other related ligands were not recognized by infected individual neurons, indicating a high degree of selectivity by the overexpressed receptor. Removal of G-protein signaling eliminates Ca responses, indicating that the endogenous second messenger system is essential for observing receptor activation. Our results provide a novel expression system for vomeronasal receptors that should be useful for understanding the molecular logic of VNO ligand detection. Functional expression of vomeronasal receptors and their deorphanization provides an essential requirement for deciphering the neural mechanisms controlling behavior.
Publisher version (URL)https://doi.org/10.1371/journal.pone.0156092
URIhttp://hdl.handle.net/10261/157863
DOI10.1371/journal.pone.0156092
Identifiersdoi: 10.1371/journal.pone.0156092
e-issn: 1932-6203
Appears in Collections:(IBGM) Artículos
Files in This Item:
File Description SizeFormat 
HSV-1derived.PDF1,95 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.