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Title: | A class-selective immunoassay for simultaneous analysis of anilinopyrimidine fungicides using a rationally designed hapten |
Authors: | Esteve Turrillas, Francesc A. ![]() ![]() ![]() |
Keywords: | Linked-Immunosorbent-Assay Chromatography-mass-spectrometry Antibody-based immunoassays Solid-phase extraction Red wine Organophosphorus pesticides Liquid-chromatography Residue analysis Grapes Pyrimethanil |
Issue Date: | 14-Sep-2017 |
Publisher: | Royal Society of Chemistry (UK) |
Citation: | Analyst 142: 3975-3985 (2017) |
Abstract: | he development of multianalyte immunoassays constitutes a main research issue in the field of bioanalytical techniques. In the present study, class-specific antibodies against the three members of the anilinopyrimidine family of fungicides (pyrimethanil, cyprodinil and mepanipyrim) were raised by using a bioconjugate of a rationally designed hapten [5-(6-methyl-2-(phenylamino)pyrimidin-4-yl)pentanoic acid]. Highly sensitive immunoassays were developed for the generic determination of these compounds, using the competitive enzyme-linked immunosorbent assay (ELISA). Particularly, a direct antibody-coated competitive ELISA afforded identical sensitivity for the three anilinopyrimidines, with IC50 values of 0.26, 0.27 and 0.25 μg L−1 for pyrimethanil, cyprodinil and mepanipyrim, respectively. This immunoassay was fully characterized and applied to the multianalyte determination of anilinopyrimidine fungicides in white and red wines, with a limit of quantification of 1 μg L−1, average recoveries from 93.1 to 114.4%, and relative standard deviations lower than 20%. Commercial wine samples were analyzed and those containing detectable anilinopyrimide residues were verified by a reference chromatographic technique. |
Publisher version (URL): | http://dx.doi.org/10.1039/c7an01138e |
URI: | http://hdl.handle.net/10261/157701 |
DOI: | http://dx.doi.org/10.1039/c7an01138e |
ISSN: | 0003-2654 |
Appears in Collections: | (IATA) Artículos |
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Analyst-2017-Esteve.pdf | Artículo principal | 672,52 kB | Adobe PDF | ![]() View/Open |
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