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Title

A class-selective immunoassay for simultaneous analysis of anilinopyrimidine fungicides using a rationally designed hapten

AuthorsEsteve Turrillas, Francesc A. ; Mercader Badia, Josep Vicent ; Agullo, Consuelo; Abad Somovilla, Antonio; Abad Fuentes, Antonio
KeywordsLinked-Immunosorbent-Assay
Chromatography-mass-spectrometry
Antibody-based immunoassays
Solid-phase extraction
Red wine
Organophosphorus pesticides
Liquid-chromatography
Residue analysis
Grapes
Pyrimethanil
Issue Date14-Sep-2017
PublisherRoyal Society of Chemistry (UK)
CitationAnalyst 142: 3975-3985 (2017)
Abstracthe development of multianalyte immunoassays constitutes a main research issue in the field of bioanalytical techniques. In the present study, class-specific antibodies against the three members of the anilinopyrimidine family of fungicides (pyrimethanil, cyprodinil and mepanipyrim) were raised by using a bioconjugate of a rationally designed hapten [5-(6-methyl-2-(phenylamino)pyrimidin-4-yl)pentanoic acid]. Highly sensitive immunoassays were developed for the generic determination of these compounds, using the competitive enzyme-linked immunosorbent assay (ELISA). Particularly, a direct antibody-coated competitive ELISA afforded identical sensitivity for the three anilinopyrimidines, with IC50 values of 0.26, 0.27 and 0.25 μg L−1 for pyrimethanil, cyprodinil and mepanipyrim, respectively. This immunoassay was fully characterized and applied to the multianalyte determination of anilinopyrimidine fungicides in white and red wines, with a limit of quantification of 1 μg L−1, average recoveries from 93.1 to 114.4%, and relative standard deviations lower than 20%. Commercial wine samples were analyzed and those containing detectable anilinopyrimide residues were verified by a reference chromatographic technique.
Publisher version (URL)http://dx.doi.org/10.1039/c7an01138e
URIhttp://hdl.handle.net/10261/157701
DOIhttp://dx.doi.org/10.1039/c7an01138e
ISSN0003-2654
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