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http://hdl.handle.net/10261/157684
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dc.contributor.author | Sacramento, Joana F. | - |
dc.contributor.author | Ribeiro, Maria J. | - |
dc.contributor.author | Yubero, Sara | - |
dc.contributor.author | Melo, Bernardete F. | - |
dc.contributor.author | Obeso, Ana | - |
dc.contributor.author | Guarino, Maria P. | - |
dc.contributor.author | González, Constancio | - |
dc.contributor.author | Conde, Silvia V. | - |
dc.date.accessioned | 2017-11-24T07:49:48Z | - |
dc.date.available | 2017-11-24T07:49:48Z | - |
dc.date.issued | 2015 | - |
dc.identifier | doi: 10.1016/j.ejps.2015.01.011 | - |
dc.identifier | issn: 0928-0987 | - |
dc.identifier | e-issn: 1879-0720 | - |
dc.identifier.citation | European Journal of Pharmaceutical Sciences 70: 107-116 (2015) | - |
dc.identifier.uri | http://hdl.handle.net/10261/157684 | - |
dc.description.abstract | Caffeine, a non-selective adenosine antagonist, has distinct effects on insulin sensitivity when applied acutely or chronically. Herein, we investigated the involvement of adenosine receptors on insulin resistance induced by single-dose caffeine administration. Additionally, the mechanism behind adenosine receptor-mediated caffeine effects in skeletal muscle was assessed. The effect of the administration of caffeine, 8-cycle-1,3-dipropylxanthine (DPCPX, A1 antagonist), 2-(2-Furanyl)-7-(2-phenylethyl)-7H-pyrazolo[4,3-e][1,2,4]triazolo[1,5-c]pyrimidin-5-amine (SCH58261, A2A antagonist) and 8-(4-{[(4-cyanophenyl)carbamoylmethyl]-oxy}phenyl)-1,3-di(n-propyl)xanthine (MRS1754, A2B antagonist) on whole-body insulin sensitivity was tested. Skeletal muscle Glut4,5′-AMP activated protein kinase (AMPK) and adenosine receptor protein expression were also assessed. The effect of A1 and A2B adenosine agonists on skeletal muscle glucose uptake was evaluated in vitro. Sodium nitroprussiate (SNP, 10 nM), a nitric oxide (NO) donor, was used to evaluate the effect of NO on insulin resistance induced by adenosine antagonists. Acute caffeine decreased insulin sensitivity in a concentration dependent manner (Emax = 55.54 ± 5.37%, IC50 = 11.61 nM), an effect that was mediated by A1 and A2B adenosine receptors. Additionally, acute caffeine administration significantly decreased Glut4, but not AMPK expression, in skeletal muscle. We found that A1, but not A2B agonists increased glucose uptake in skeletal muscle. SNP partially reversed DPCPX and MRS1754 induced-insulin resistance. Our results suggest that insulin resistance induced by acute caffeine administration is mediated by A1 and A2B adenosine receptors. Both Glut4 and NO seem to be downstream effectors involved in insulin resistance induced by acute caffeine. | - |
dc.description.sponsorship | This study was supported by the Portuguese Foundation for Science and Technology Grant PTDC/SAU-ORG/111417/2009 to Silvia V. Conde and by Grants BFU2012-37459 from the Spanish Ministry of Economy and Competitiveness, and CIBER CB06/06/0050 from the Spanish Ministry of Health-Institute Carlos III to Constancio Gonzalez. | - |
dc.publisher | Elsevier | - |
dc.rights | closedAccess | - |
dc.subject | Nitric oxide | - |
dc.subject | Glut4 | - |
dc.subject | Insulin resistance | - |
dc.subject | Caffeine | - |
dc.subject | Adenosine receptors | - |
dc.title | Disclosing caffeine action on insulin sensitivity: Effects on rat skeletal muscle | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1016/j.ejps.2015.01.011 | - |
dc.date.updated | 2017-11-24T07:49:48Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.contributor.funder | Fundação para a Ciência e a Tecnologia (Portugal) | - |
dc.contributor.funder | Instituto de Salud Carlos III | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004587 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100001871 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.fulltext | No Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairetype | artículo | - |
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