English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/157671
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Characterization of resistance against the olive-defoliating Verticillium dahliae pathotype in selected clones of wild olive

AutorJiménez Fernández, Daniel; Trapero Casas, José Luis ; Landa, Blanca B. ; Navas Cortés, Juan Antonio ; Bubici, Giovanni; Cirulli, Matteo; Jiménez-Díaz, Rafael M.
Palabras claveOlea europaea L. subsp. europaea var. sylvestris
Olive
qPCR
Rootstocks
Verticillium wilt
Fecha de publicaciónoct-2016
EditorJohn Wiley & Sons
CitaciónPlant Pathology 65(8): 1279-1291 (2016)
ResumenVerticillium wilt of olive is best managed by resistant cultivars, but those currently available show incomplete resistance to the defoliating (D) Verticillium dahliae pathotype. Moreover, these cultivars do not satisfy consumers' demand for high yields and oil quality. Highly resistant rootstocks would be of paramount importance for production of agronomically adapted and commercially desirable olive cultivars in D V. dahliae-infested soils. In this work, resistance to D V. dahliae in wild olive clones Ac-13, Ac-18, OutVert and StopVert was assessed by quantifying the fungal DNA along the stem using a highly sensitive real-time quantitative polymerase chain reaction (qPCR) protocol and a stem colonization index (SCI) based on isolation of V. dahliae following artificial inoculations under conditions highly conducive for verticillium wilt. Ac-13, Ac-18, OutVert and StopVert showed a symptomless reaction to D V. dahliae. The mean amount of D V. dahliaeDNA quantified in stems of the four clones ranged from 3.64 to 28.89 pg/100 ng olive DNA, which was 249 to 1537 times lower than that in susceptible Picual olive. The reduction in the quantitative stem colonization of wild olive clones by D V. dahliae was also indicated by a sharp decrease in the SCI. Overall, there was a pattern of decreasing SCI in acropetal progression along the plant axis, as well as correlation between positive reisolation and quantification of pathogen DNA. The results of this research show that wild olive clones Ac-13, Ac-18, OutVert and StopVert have a valuable potential as rootstocks for the management of verticillium wilt in olive.
Versión del editorhttp://doi.org/10.1111/ppa.12516
URIhttp://hdl.handle.net/10261/157671
DOI10.1111/ppa.12516
ISSN0032-0862
E-ISSN1365-3059
Aparece en las colecciones: (IAS) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.