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Fission yeast cell cycle synchronization methods

AuthorsTormos-Pérez, Marta; Pérez-Hidalgo, Livia; Moreno, Sergio
KeywordsCentrifugal elutriation
Block and release
Schizosaccharomyces pombe
Fission yeast
Lactose gradient
S-phase G2-phase
Issue Date2016
PublisherSpringer Nature
CitationYeast Cytokinesis. Methods and Protocols: 293-308 (2016)
SeriesMethods in Molecular Biology 1369
AbstractFission yeast cells can be synchronized by cell cycle arrest and release or by size selection. Cell cycle arrest synchronization is based on the block and release of temperature-sensitive cell cycle mutants or treatment with drugs. The most widely used approaches are cdc10-129 for G1; hydroxyurea (HU) for early S-phase; cdc25-22 for G2, and nda3-KM311 for mitosis. Cells can also be synchronized by size selection using centrifugal elutriation or a lactose gradient. Here we describe the methods most commonly used to synchronize fission yeast cells.
Identifiersisbn: 978-1-4939-3144-6
Appears in Collections:(IBFG) Libros y partes de libros
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