English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/157486
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Radioactive labeling and fractionation of fission yeast walls

AuthorsPérez, Pilar; Ribas, Juan Carlos
Issue Date2016
PublisherCold Spring Harbor Laboratory Press
CitationFission Yeast: A laboratory manual: 395-402 (2016)
AbstractFungal cells contain an essential structure external to the cell, made of polysaccharides and proteins, termed the cell wall. Polysaccharides represent ∼96% of the cell wall on a dry-weight basis. They are complex insoluble polymers connected to each other by covalent linkages and hydrogen bonds with specific localizations in the cell wall and septum. Fission yeast contains three β-glucans (a major branched β(1,3)-glucan, a minor linear β(1,3)-glucan, and a minor branched β(1,6)-glucan), two α-glucans (a major α(1,3)-glucan and a minor α(1,4)-glucan), and a minor amount of galactomannan-linked glycoproteins. We provide here a simple protocol to label uniformly the cell wall using D-[U-14C]-glucose as a carbon source and to fractionate the cell wall into the three or four main cell wall components: galactomannoproteins, α-glucan, and β-glucan, which can be subdivided into β(1,3)-glucan and β(1,6)-glucan. This simple protocol uses enzymatic and chemical fractionations of the different cell wall components that permit the quantification of each polysaccharide in the cell wall and in the cell. This protocol is very useful for the analysis of the many morphological alterations caused by a variety of cellular processes that ultimately affect the cell wall and thus cell morphogenesis.
Identifiersisbn: 978-1-621820-82-6
Appears in Collections:(IBFG) Libros y partes de libros
Files in This Item:
File Description SizeFormat 
Radioactivelabeling.pdf424 kBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.