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Title

Fission yeast cell wall analysis

AuthorsPérez, Pilar; Ribas, Juan Carlos
Issue Date2016
PublisherCold Spring Harbor Laboratory. Press
CitationFission Yeast: A laboratory manual: 387-402 (2016)
AbstractThe Schizosaccharomyces pombe cell wall is a rigid exoskeletal structure mainly composed of interlinked glucose polysaccharides and galactomannoproteins. It is essential for survival of the fission yeast, as it prevents cells from bursting from internal turgor pressure and protects them from mechanical injuries. Additionally, the cell wall determines the cell shape and, therefore, a better knowledge of cell wall structure and composition could provide valuable data in S. pombe morphogenetic studies. Here, we provide information about this structure and the current reliable methods for rapid analysis of the cell wall polymers by specific enzymatic and chemical degradations of purified cell walls.
URIhttp://hdl.handle.net/10261/157482
Identifiersisbn: 978-1-621820-82-6
Appears in Collections:(IBFG) Libros y partes de libros
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