English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/157407
Título

p27Kip1 participates in the regulation of endoreplication in differentiating chick retinal ganglion cells

AutorOvejero-Benito, M. C. ; Frade López, José María
Palabras claveCell cycle
Endocycle
Endoreduplication
Neurogenesis
Polyploidy
Retina
RGC
Vertebrate
Fecha de publicación6-may-2015
EditorTaylor & Francis
CitaciónCell Cycle 14(14) : 2311-2322 (2015)
ResumenNuclear DNA duplication in the absence of cell division (i.e. endoreplication) leads to somatic polyploidy in eukaryotic cells. In contrast to some invertebrate neurons, whose nuclei may contain up to 200,000-fold the normal haploid DNA amount (C), polyploid neurons in higher vertebrates show only 4C DNA content. To explore the mechanism that prevents extra rounds of DNA synthesis in these latter cells we focused on the chick retina, where a population of tetraploid retinal ganglion cells (RGCs) has been described. We show that differentiating chick RGCs that express the neurotrophic receptors p75 and TrkB while lacking retinoblastoma protein, a feature of tetraploid RGCs, also express p27Kip1. Two different short hairpin RNAs (shRNA) that significantly downregulate p27Kip1 expression facilitated DNA synthesis and increased ploidy in isolated chick RGCs. Moreover, this forced DNA synthesis could not be prevented by Cdk4/6 inhibition, thus suggesting that it is triggered by a mechanism similar to endoreplication. In contrast, p27Kip1 deficiency in mouse RGCs does not lead to increased ploidy despite previous observations have shown ectopic DNA synthesis in RGCs from p27Kip1−/− mice. This suggests that a differential mechanism is used for the regulation of neuronal endoreplication in mammalian versus avian RGCs.
Versión del editorhttp://dx.doi.org/10.1080/15384101.2015.1044175
URIhttp://hdl.handle.net/10261/157407
DOI10.1080/15384101.2015.1044175
ISSN1538-4101
Aparece en las colecciones: (IC) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
p27Kip1 participates in the regulation.pdf1,26 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.