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How do budding yeast cells coordinate late cytokinesis steps?

AuthorsFoltman, Magdalena ; Molist, Iago; Arcones, Irene; Sacristán, Carlos; Roncero, Cesar; Sánchez-Díaz, Alberto
Issue Date2015
Citation10ª Reunión de la Red Española de Levaduras (2015)
AbstractDuring cytokinesis, cells coordinate contraction of the actomyosin ring with the ingression of the plasma membrane and remodelling of the extracellular matrix (ECM) but the underlying mechanisms are still poorly understood. In eukaryotes, glycosyltransferases that synthesise ECM polysaccharides are emerging as important players during cytokinesis. In budding yeast the chitin synthase Chs2 makes the primary septum, a special layer of ECM that is essential for cell division. To try to understand how yeast cells coordinate actomyosin ring contraction, plasma membrane ingression and remodelling of the extracellular matrix we have used budding yeast Chs2 and Inn1 proteins to isolate 'ingression progression complexes' (IPCs) that contain key actomyosin rings components. We have identified, together with Chs2 and Inn1, actomyosin ring components Cyk3, myosin type II, the IQGAP protein Iqg1 and Hof1. We propose that IPCs are central to the mechanism by which cells coordinate cytokinesis. We have found that the catalytic domain of Chs2 interacts directly with the C2 domain of Inn1 and the transglutaminase-like domain of Cyk3. We have now data indicating that Inn1, Chs2 and Cyk3 form a stable complex. We found that chitin synthase Chs2 is activated by C2 domain of Inn1, as well as the transglutaminase-like domain of Cyk3. On the other hand, it has been already described that Chs2 protein is transported in vesicles to the cleavage site at the end of mitosis, but the molecular mechanisms by which those vesicles carrying Chs2 are incorporated at the site of division are unknown. We are now characterizing some of the factors we identified interacting with IPCs during cytokinesis that could have a specific role in that process.
DescriptionResumen del trabajo presentado a la 10ª Reunión de la Red Española de Levaduras, celebrada en El Escorial (Madrid) del 16 al 18 de diciembre de 2015.
Appears in Collections:(IBFG) Comunicaciones congresos
(IBBTEC) Comunicaciones congresos
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