English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/157250
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Arf6p regulates endocytosis and morphogenesis in Schizosaccharomyces pombe

AutorYanguas, Francisco; León, Nagore de; Hoya, Marta; Curto, María Ángeles; Moro, Sandra; Doncel, Cristina; Valdivieso, María Henar
Fecha de publicación2015
CitaciónYLS Symposium (2015)
ResumenIntracellular protein transport requires the generation and traffic of coated vesicles that originate in, and fuse to organelle membranes. The correct timing and directionality of this transport is guaranteed by guanosine tri-phosphate hydrolases (GTPases) nd phosphoinositides, which contribute to organelle identity. In metazoa, Arf6p participates in clathrin-mediated and clathrin-independemt endocytosis, and has been suggested to participate in traffic through endosomes. On the contrary, no direct evidence of the involvement of this GTPase in endocytosis has been found in unciellular eukaryotes. we have found that in fission yeast arf6Δ mutants interact genetically with mutants in clc1+ and end4+, and that the absence of Arf6p produces a defect in the behavior of the endocytic patches. Additionally, apl3Δ (defective in the clathrin adaptor AP2) and arf6Δ mutants are defective in cell wall synthesis, sterol distribution at the plasma membrane, and polarity. All phenotypes are severly aggravated in double apl3Δ arf6Δ mutant. The severity of these phenotypes correlates with the defect in the dynamics of the endocytic patches. These results show that the participation of Arf6p in endocytosis has been conserved along evolution, and suggest that Arf6p might play AP2-independent roles in vesicle trafficking and morphogenesis. As a tool for the future analysis of these roles, several fluorescent phosphoinositide-binding probes have been constructed. Also GFP-tagged phosphoinositide kinases and phosphatases are under construction.
DescripciónResumen del póster presentado al Young Life Scientists’ Symposium: "Cell cycle: the challenge to coordinate genome stability and morphogenesis", celebrado en Salamanca (España) el 4 de diciembre del 2015.
URIhttp://hdl.handle.net/10261/157250
Aparece en las colecciones: (IBFG) Comunicaciones congresos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.