Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/157179
COMPARTIR / EXPORTAR:
SHARE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Herrera, Carlos M. | es_ES |
dc.contributor.author | Alonso, Conchita | es_ES |
dc.contributor.author | Medrano, Mónica | es_ES |
dc.contributor.author | Pérez, Ricardo | es_ES |
dc.contributor.author | Bazaga, Pilar | es_ES |
dc.coverage.spatial | Cuevas Bermejas: (Longitude º W / Latitude º N) 2.85153 / 37.96558, altitude: 1185 m asl | es_ES |
dc.date.accessioned | 2017-11-10T07:50:00Z | - |
dc.date.available | 2017-11-10T07:50:00Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Herrera, Carlos M. ; Alonso, Conchita ; Medrano, Mónica ; Pérez, Ricardo ; Bazaga, Pilar; 2017; "Global cytosine methylation, genetic markers (AFLP) and epigenetic markers (MSAP) in 15 maternal plants of the shrub Lavandula latifolia (Lamiaceae) and their offspring [Dataset]"; DIGITALCSIC; http://dx.doi.org/10.20350/digitalCSIC/8526 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/157179 | - |
dc.description | This contains three Microsoft Excel files for global methylation, AFLP and MSAP data, respectively. On each file one sheet contains the information of maternal plants and the other the information of the offspring. Each row refers to individual samples. For AFLP and MSAP each column refers to specific (although anonymous) markers labeled similarly in both sheets. Leaves from 15 adult plants were sampled along a 125-m transect in Cuevas Bermejas population (Sierra de Cazorla, Jaén Province, southeastern Spain), in October 2015. Offspring were obtained from seeds under greenhouse conditions, leaf samples were collected when they were 8-months old when only offspring of 13 mothers remained. DNA was extracted from leaves using Qiagen DNeasy Plant Mini Kit and the manufacturer protocol. Maternal parents and greenhouse plants were genetically fingerprinted using amplified fragment polymorphism (AFLP) markers. The AFLP and MSAP analysis were performed using standard protocols involving the use of fluorescent dye-labeled selective primers. For AFLP, each plant was fingerprinted using three different EcoRI + 3 / MseI + 3 primer pair combinations. MSAP assays used four HpaII-MspI + 2 / MseI + 3 primer combinations. In the two analyses fragment separation and detection was made using an ABI PRISM 3130xl DNA sequencer, only fragments ≥ 150 base pairs in size were considered and the presence (1) or absence (0) of fragments in each sample was scored manually by visualizing electropherograms with GeneMapper 3.7 software | es_ES |
dc.description.abstract | This dataset includes (i) global DNA cytosine methylation estimates (= percentage of all genomic cytosines that are methylated) obtained by reverse phase HPLC with spectrofluorimetric detection, (ii) the scoring (presence/absence) of individual genetic markers (AFLP), and (iii) the scoring (presence/absence) of individual epigenetic markers (MSAP) in maternal plants of the shrub Lavandula latifolia and their greenhouse-grown offspring. | es_ES |
dc.language.iso | eng | es_ES |
dc.relation.isreferencedby | Herrera, Carlos M.; Alonso, Conchita; Medrano, Mónica; Pérez, Ricardo; Bazaga, Pilar. Transgenerational epigenetics: inheritance of global cytosine methylation and methylation-related epigenetic markers in the shrub. Lavandula latifolia. American Journal of Botany. http://dx.doi.org/10.1002/ajb2.1074 | - |
dc.rights | openAccess | es_ES |
dc.subject | Amplified fragment length polymorphisms (AFLP) | es_ES |
dc.subject | Epigenetic variation | es_ES |
dc.subject | Global DNA methylation | es_ES |
dc.subject | HPLC | es_ES |
dc.subject | Inheritance | es_ES |
dc.subject | Lavandula latifolia | es_ES |
dc.subject | Methylation-sensitive amplified fragment length polymorphisms (MSAP) | es_ES |
dc.subject | Spain | es_ES |
dc.title | Global cytosine methylation, genetic markers (AFLP) and epigenetic markers (MSAP) in 15 maternal plants of the shrub Lavandula latifolia (Lamiaceae) and their offspring | es_ES |
dc.type | dataset | es_ES |
dc.identifier.doi | 10.20350/digitalCSIC/8526 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.rights.license | http://creativecommons.org/licenses/by-nc-sa/4.0/ | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.contributor.contactPerson | Herrera, Carlos M. <herrera@ebd.csic.es> | - |
dc.type.coar | http://purl.org/coar/resource_type/c_ddb1 | es_ES |
item.openairetype | dataset | - |
item.cerifentitytype | Products | - |
item.languageiso639-1 | en | - |
item.grantfulltext | open | - |
item.openairecristype | http://purl.org/coar/resource_type/c_ddb1 | - |
item.fulltext | With Fulltext | - |
Aparece en las colecciones: | (EBD) Conjuntos de datos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Maternal.parents.and.offspring.AFLP.xlsx | 53,28 kB | Microsoft Excel XML | Visualizar/Abrir | |
Maternal.parents.and.offspring.methylation.xlsx | 14,34 kB | Microsoft Excel XML | Visualizar/Abrir | |
Maternal.parents.and.offspring.MSAP.xlsx | 101,18 kB | Microsoft Excel XML | Visualizar/Abrir | |
readme_global.txt | 3,1 kB | Text | Visualizar/Abrir |
CORE Recommender
Page view(s)
660
checked on 27-mar-2024
Download(s)
266
checked on 27-mar-2024
Google ScholarTM
Check
Altmetric
Altmetric
Este item está licenciado bajo una Licencia Creative Commons