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Traffic between Golgi, endosomes and prevacuolar compartment: a role for the exomer?

AutorHoya, Marta; Yanguas, Francisco; León, Nagore de; Doncel, Cristina; Valdivieso, María Henar
Fecha de publicación2015
CitaciónYLS Symposium (2015)
ResumenIn the budding yeast Saccharomyces cerevisiae a protein complex termed exomer has been shown to be required for the delivery of certain transmembrane proteins to the cell surface. The exomer is composed od Chs5p and four ChAPs (Chs5 and Arf-bindinig proteins). Chs5p is reuired for the localization of the complex at the Trans-Golgi-Network (TGN). The best characterized cargo is the chitin synthase Chs3p. In Schizosaccharomyces pombe there are no homologues to Chs3p and glucans are the main components of the cell wall. In this fission yeast Cfr1p is similar to Chs5p, and Bch1p is the only protein similar tl the ChAPs. Cfr1p and Bch1p co-immunoprecipitate, co-localize, and are interdependent for their localization. Cfr1 co-localizes with Sec72 but not with Anp1p. Additionally, 30% of Cfr1p co-fractionates with prevacuolar compartment (PVC) and vacuole markers. We have found co-immunoprecipitation between Cfr1p and AP-1 and AP-2 components, and genetic interaction with mutants in the AP-1, AP-2, and GGA clathrin adaptors. No relationship with AP-3, COPI and COPII components has been found. In cfr1Δ mutants, co-migration of the β(1,3)glucan synthases Bgs1p and Bgs4p accumulates in structures that might correspond to early endosomes, and in the double cfr1Δ apm 1Δ mutants this abnormal accumulation is enhanced. While these aggregates are partially resolved by latrunculin in the apm1Δ mutant, they are not resolved in the cfr1Δ apm 1Δ mutant. Finally, in the cfr1Δ, bch1Δ, and cfr1Δ bch1Δ strains, the vacuolar carboxypeptidase Y is missorted to the cell surface. These results show that the fission yeast exomer plays a pervasive role in the traffic between the Golgi and the endosomal and prevacuolar compartments.
DescripciónResumen del póster presentado al Young Life Scientists’ Symposium: "Cell cycle: the challenge to coordinate genome stability and morphogenesis", celebrado en Salamanca (España) el 4 de diciembre del 2015.
Aparece en las colecciones: (IBFG) Comunicaciones congresos
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