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Monitoring chitin deposition during septum assembly in budding yeast

AuthorsArcones, Irene; Roncero, Cesar
Primary septum
Issue Date2016
PublisherSpringer Nature
CitationYeast Cytokinesis: 59-72 (2016)
SeriesMethods in Molecular Biology 1369
AbstractThe synthesis of the septum is a critical step during cytokinesis in the fungal cell. Moreover, in Saccharomyces cerevisiae septum assembly depends mostly on the proper synthesis and deposition of chitin and, accordingly, on the timely regulation of chitin synthases. In this chapter, we will see how to follow chitin synthesis by two complementary approaches: monitoring chitin deposition in vivo at the septum by calcofluor staining and fluorescence microscopy, and measuring the chitin synthase activities responsible for this synthesis.
Publisher version (URL)https://doi.org/10.1007/978-1-4939-3145-3_5
Identifiersdoi: 10.1007/978-1-4939-3145-3_5
isbn: 978-1-4939-3144-6
Appears in Collections:(IBFG) Libros y partes de libros
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