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dc.contributor.authorPérez, Pilar-
dc.contributor.authorPortales, Elvira-
dc.contributor.authorSantos Romero, Beatriz-
dc.identifierdoi: 10.1099/mic.0.000062-
dc.identifierissn: 1350-0872-
dc.identifiere-issn: 1465-2080-
dc.identifier.citationMicrobiology 161(5): 948-959 (2015)-
dc.description.abstractRho GTPases are small proteins present in all eukaryotic cells, from yeast to mammals, with a function in actin organization and morphogenetic processes. Schizosaccharomyces pombe Rho4 is not essential but it displays a role during cell separation at high temperature. In fact, Rho4 is involved in the secretion of the hydrolytic enzymes that are required for cell septum degradation during this process. In rho4¿ cells, vesicles accumulate in the septum area and the glucanases Eng1 and Agn1 are not secreted to the culture medium. The localization of Eng1 and Agn1 depends on the exocyst and the septins. The exocyst is a conserved multiprotein complex important for the targeting and fusion of Golgi-derived vesicles with the plasma membrane. Septins are a family of GTP-binding proteins conserved in eukaryotes that function during cytokinesis. Here we show that Rho4 is required for the proper localization of the exocyst and septins at high temperature. Moreover, pull-down experiments demonstrate that Rho4 can interact with exocyst subunits, such as Sec8 and Exo70, and septin proteins, such as Spn3. We observe that Sec8 preferentially binds to activated GTP-Rho4, suggesting that Sec8 could be an effector of this GTPase. We propose that the interaction of Rho4 with the exocyst and septins confers a precise regulation for the secretion of glucanases at the appropriate place and time during the cell cycle.-
dc.description.sponsorshipThis work was supported by Dirección General de Investigación, MICINN, Spain (grant BFU2013-39394P) and by Junta de Castilla y León (grant CSI037U14).-
dc.publisherSociety for General Microbiology-
dc.titleRho4 interaction with exocyst and septins regulates cell separation in fission yeast-
dc.description.versionPeer Reviewed-
dc.contributor.funderMinisterio de Ciencia e Innovación (España)-
dc.contributor.funderJunta de Castilla y León-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
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