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Título

The first cytochrome P450 in ferns. Evidence for its involvement in phytoecdysteroid biosynthesis in Polypodium vulgare

AutorCanals, Daniel; Irurre-Santilari, Josep; Casas Brugulat, Josefina
Palabras claveCytochrome P450
Ferns
Phytoecdysteroids
Sterol pathways
Fecha de publicación6-sep-2005
EditorBlackwell Publishing
CitaciónFEBS Journal 272(18): 4817-4825 (2005)
ResumenThe fern Polypodium vulgare is a phytoecdysteroid (PE)-producing plant. Cultures of P. vulgare prothalus produce PE, whereas prothalus-derived callus cultures do not. However, this callus line can transform topically applied ecdysone (E) to 20-hydroxyecdysone (20E), which is the last step in the biosynthetic pathway of the main plant PE. This hydroxylation is catalysed by a cytochrome P450 enzyme. E treatment of the callus line results in an increased amount of P450, showing a linear correspondence between the amount of P450 and in vivo E 20-hydroxylation activity, estimated by measuring the bioconversion of E to 20E. This activity can be inhibited by molecules that bind to the P450-heme group. E shows a P450-substrate-binding spectrum with microsomes that overexpress the P450 protein. Finally, a P450 protein was purified from E-treated calli, this being the first P450 to be described in the pterydophyte group.
Descripción9 pages, 7 figures, 1 table.-- PMID: 16156800 [PubMed].-- Printed version published Sep 2005.
Versión del editorhttp://dx.doi.org/10.1111/j.1742-4658.2005.04897.x
URIhttp://hdl.handle.net/10261/15662
DOI10.1111/j.1742-4658.2005.04897.x
ISSN1742-464X (Print)
1742-4658 (Online)
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