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The RNA-binding protein Tb DRBD3 regulates the stability of a specific subset of mRNAs in trypanosomes

AuthorsEstévez, Antonio M.
Keywordsrna, messenger
rna-binding proteins
rna interference
Issue Date8-Jul-2008
PublisherOxford University Press
CitationNucleic Acids Research
AbstractIn trypanosomes, the apparent lack of regulation of RNA polymerase II-dependent transcription initiation poses a challenge to understand how these eukary- otes adjust gene expression to adapt to the con- trasting environments they find during their life cycles. Evidence so far indicates that mRNA turnover and translation are the major control points in which regulation is exerted in trypanosomes. However, very little is known about which proteins are involved, and how do they regulate the abundance and translation of different mRNAs in different life stages. In this work, an RNA-binding protein, Tb DRBD3, has been identified by affinity chromatography, and its func- tion addressed using RNA interference, microarray analysis and immunoprecipitation of mRNA–protein complexes. The results obtained indicate that Tb DRBD3 binds to a subset of developmentally regu- lated mRNAs encoding membrane proteins, and that this association promotes the stabilization of the target transcripts. These observations raise the pos- sibility that Tb DRBD3-mRNA complexes act as a post-transcriptional operon, and provide a frame- work to interpret how trypanosomes regulate gene expression in the absence of transcriptional control.
Publisher version (URL)https://academic.oup.com/nar/article/36/14/4573/2410220/The-RNA-binding-protein-Tb-DRBD3-regulates-the
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