English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/156254
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Vitronectin regulates Sonic hedgehog activity during cerebellum development through CREB phosphorylation

AutorPons, Sebastián ; Trejo, José L. ; Martínez-Morales, Juan Ramón ; Martí, E.
Palabras claveCyclic AMP responsive element-binding protein (CREB), phosphorylated-CREB (P-CREB), CREB-binding-protein (CBP)
Vitronectin
Laminin
Extracellular matrix glycoproteins
Embryo
Purkinje neuron
Proliferation, Differentiation
Chick, Rat
Cerebellum
Granule neuron
Fecha de publicación2001
EditorCompany of Biologists
CitaciónDevelopment 128: 1481- 1492 (2001)
ResumenDuring development of the cerebellum, Sonic hedgehog (SHH) is expressed in migrating and settled Purkinje neurons and is directly responsible for proliferation of granule cell precursors in the external germinal layer. We have previously demonstrated that SHH interacts with vitronectin in the differentiation of spinal motor neurons. Here, we analysed whether similar interactions between SHH and extracellular matrix glycoproteins regulate subsequent steps of granule cell development. Laminins and their integrin receptor subunit α6 accumulate in the outer most external germinal layer where proliferation of granule cell precursors is maximal. Consistent with this expression pattern, laminin significantly increases SHH-induced proliferation in primary cultures of cerebellar granule cells. Vitronectin and its integrin receptor subunits αv are expressed in the inner part of the external germinal layer where granule cell precursors exit the cell cycle and commence differentiation. In cultures, vitronectin is able to overcome SHH-induced proliferation, thus allowing granule cell differentiation. Our studies indicate that the pathway in granule cell precursors responsible for the conversion of a proliferative SHH-mediated response to a differentiation signal depends on CREB. Vitronectin stimulates phosphorylation of cyclic-AMP responsive element-binding protein (CREB), and over-expression of CREB is sufficient to induce granule cell differentiation in the presence of SHH. Taken together, these data suggest that granule neuron differentiation is regulated by the vitronectin-induced phosphorylation of CREB, a critical event that terminates SHH-mediated proliferation and permits the differentiation program to proceed in these cells.
URIhttp://hdl.handle.net/10261/156254
DOInull
Identificadoresdoi: null
issn: 0950-1991
Aparece en las colecciones: (IC) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Development.pdf1,38 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.