English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/156026
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Persulfidation proteome reveals the regulation of protein function by hydrogen sulfide in diverse biological processes in Arabidopsis

AutorAroca, Ángeles ; Benito, Juan M. ; Gotor, Cecilia ; Romero, Luis C.
Palabras claveCisteine
Hydrogen suldife
Mass spectrometry
Persulfidation
Post-translational modification
Proteomics
Fecha de publicación2017
EditorOxford University Press
CitaciónJournal of Experimental Botany, erx294 (2017)
ResumenHydrogen sulfide-mediated signaling pathways regulate many physiological and pathophysiological processes in mammalian and plant systems. The molecular mechanism by which hydrogen sulfide exerts its action involves the post-translational modification of cysteine residues to form a persulfidated thiol motif, a process called protein persulfidation. We have developed a comparative and quantitative proteomic analysis approach for the detection of endogenous persulfidated proteins in wild-type Arabidopsis and L-CYSTEINE DESULFHYDRASE 1 mutant leaves using the tag-switch method. The 2015 identified persulfidated proteins were isolated from plants grown under controlled conditions, and therefore, at least 5% of the entire Arabidopsis proteome may undergo persulfidation under baseline conditions. Bioinformatic analysis revealed that persulfidated cysteines participate in a wide range of biological functions, regulating important processes such as carbon metabolism, plant responses to abiotic and biotic stresses, plant growth and development, and RNA translation. Quantitative analysis in both genetic backgrounds reveals that protein persulfidation is mainly involved in primary metabolic pathways such as the tricarboxylic acid cycle, glycolysis, and the Calvin cycle, suggesting that this protein modification is a new regulatory component in these pathways
Versión del editorhtpp://dx.doi.org/10.1093/jxb/erx294
URIhttp://hdl.handle.net/10261/156026
DOI10.1093/jxb/erx294
Aparece en las colecciones: (IBVF) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
watermark.pdf1,07 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.