Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/155979
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

NADPH thioredoxin reductase C and thioredoxins act concertedly in seedling development

AuthorsOjeda, Valle; Pérez-Ruiz, Juan Manuel CSIC ; González, Maricruz CSIC ORCID ; Nájera, Victoria A.; Sahrawy, Mariam; Serrato, Antonio Jesús; Geigenberger, P.; Cejudo, Francisco Javier CSIC ORCID
Issue Date2017
PublisherAmerican Society of Plant Biologists
CitationPlant Physiology 174: 1436- 1448 (2017)
AbstractThiol-dependent redox regulation of enzyme activity plays a central role in the rapid acclimation of chloroplast metabolism to ever-fluctuating light availability. This regulatory mechanism relies on ferredoxin reduced by the photosynthetic electron transport chain, which fuels reducing power to thioredoxins (Trxs) via a ferredoxin-dependent Trx reductase. In addition, chloroplasts harbor an NADPH-dependent Trx reductase, which has a joint Trx domain at the carboxyl terminus, termed NTRC. Thus, a relevant issue concerning chloroplast function is to establish the relationship between these two redox systems and its impact on plant development. To address this issue, we generated Arabidopsis (Arabidopsis thaliana) mutants combining the deficiency of NTRC with those of Trxs f, which participate in metabolic redox regulation, and that of Trx x, which has antioxidant function. The ntrc-trxf1f2 and, to a lower extent, ntrc-trxx mutants showed severe growth-retarded phenotypes, decreased photosynthesis performance, and almost abolished light-dependent reduction of fructose-1,6-bisphosphatase. Moreover, the combined deficiency of both redox systems provokes aberrant chloroplast ultrastructure. Remarkably, both the ntrc-trxf1f2 and ntrc-trxx mutants showed high mortality at the seedling stage, which was overcome by the addition of an exogenous carbon source. Based on these results, we propose that NTRC plays a pivotal role in chloroplast redox regulation, being necessary for the activity of diverse Trxs with unrelated functions. The interaction between the two thiol redox systems is indispensable to sustain photosynthesis performed by cotyledons chloroplasts, which is essential for early plant development.
URIhttp://hdl.handle.net/10261/155979
DOI10.1104/pp.17.00481
Identifiersdoi: 10.1104/pp.17.00481
issn: 0032-0889
Appears in Collections:(IBVF) Artículos

Files in This Item:
File Description SizeFormat
1436.full.pdf2,3 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work

PubMed Central
Citations

16
checked on Jan 12, 2022

SCOPUSTM   
Citations

33
checked on Jan 20, 2022

WEB OF SCIENCETM
Citations

31
checked on Jan 21, 2022

Page view(s)

347
checked on Jan 23, 2022

Download(s)

235
checked on Jan 23, 2022

Google ScholarTM

Check

Altmetric

Dimensions


Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.