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Rapid Py-GC/MS assessment of the structural alterations of lignins in genetically modified plants

AuthorsRencoret, Jorge ; Río Andrade, José Carlos del ; Nierop, Klaas G. J.; Gutiérrez Suárez, Ana ; Ralph, John
Whole cell-walls
Transgenic plants
Issue DateSep-2016
CitationJournal of Analytical and Applied Pyrolysis (121): 155–164 (2016)
AbstractGenetic modifications for perturbing the lignin pathway in three different species of angiosperm plants, including non-woody (Arabidopsis and alfalfa) and woody (poplar) plants, were readily evaluated by analytical pyrolysis coupled to gas chromatography-mass spectrometry (Py-GC/MS). Pyrolysis showed that the composition of Arabidopsis plants was severely altered when the expression of the gene encoding the enzyme caffeic acid O-methyltransferase (COMT) was downregulated, resulting in a lignin largely enriched in guaiacyl (G) units (88%). Alfalfa plants in which lignin biosynthesis was modified by down-regulation of the p-coumarate 3-hydroxylase (C3H) gene, showed extremely high proportions of p-hydroxyphenyl (H) units (71%) relative to the naturally prevailing guaiacyl (G) and syringyl (S) units. Finally, Py-GC/MS analyses indicated that overexpression in poplar of the gene that encodes the enzyme ferulate 5-hydroxylase (F5H) resulted in a lignin with a higher content of syringyl lignin units (88%) compared to the wild-type control (71%). In conclusion, Py-GC/MS is a useful and convenient tool for the rapid evaluation of compositional changes in lignin from genetically modified plants.
Description10 páginas.-- 5 figuras.-- 1 tablas.-- 45 referencias
Publisher version (URL)https://doi.org/10.1016/j.jaap.2016.07.016
Appears in Collections:(IRNAS) Artículos
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