Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/155879
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Cationic nioplexes-in-polysaccharide-based hydrogels as versatile biodegradable hybrid materials to deliver nucleic acids |
Autor: | Grijalvo, Santiago CSIC ORCID; Alagia, Adele CSIC; Puras, Gustavo; Zárate, Jon; Mayr, Judith; Pedraz, José Luís; Eritja Casadellà, Ramón CSIC ORCID ; Díaz Díaz, David CSIC ORCID | Palabras clave: | Nucleic acids Nioplexes |
Fecha de publicación: | 31-ago-2017 | Editor: | Royal Society of Chemistry (UK) | Citación: | Journal of materials Chemistry B | Resumen: | Two polysaccharide-based hydrogels made of only κ-carrageenan (4%; w/v) or of a mixture of methylcellulose:κ-carrageenan (2%; w/v) were used to encapsulate cationic nioplexes. These vesicular particles were made of a synthetic aminolipid and polysorbate-80 (Tween-80), as a non-ionic surfactant agent. According to oscillatory rheological measurements, the presence of nioplexes did not compromise the mechanical integrity of the gels. In vitro niosomal release experiments demonstrated the liberation of nioplexes up to 24 h, and the curves were fitted according to Higuchi, Korsmeyer–Peppas and Weibull equation models, which indicated Fickian-diffusion controlled mechanisms. Besides nioplexes, cervical cancer cells were also entrapped within the biohydrogels. Cell release confirmed that these materials did not affect the cell viability, allowing cells to spread and proliferate after 24 h. The applicability of these biocompatible hydrogels was also extended to gene delivery. In this regard, the best silencing activities were found when cationic niosomes were complexed with antisense oligonucleotides in KC hydrogels. Nioplexes were able to release through the hydrogel and promoted silencing of luciferase expression in the presence of serum without using commercially available cationic lipids. Overall, the formation of such hybrid materials by integrating cationic nioplexes within biodegradable hydrogels provides a new perspective for the delivery of macromolecular therapeutics. | Versión del editor: | 10.1039/C7TB01691C | URI: | http://hdl.handle.net/10261/155879 | DOI: | 10.1039/C7TB01691C |
Aparece en las colecciones: | (IQAC) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Cationic nioplexes-in-polysaccharide-based hydrogels.pdf | 1,98 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
WEB OF SCIENCETM
Citations
9
checked on 22-feb-2024
Page view(s)
300
checked on 22-abr-2024
Download(s)
422
checked on 22-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.