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Title

The transposable element Bari-Jheh mediates oxidative stress response in Drosophila

AuthorsGuio, Lain; Barrón, Maite G. ; González Pérez, Josefa
Issue DateMay-2014
CitationSystems Genetics and Evolution of non-human (model) organisms (2014)
AbstractElucidating the fitness effects of natural genetic variants is one of the current major challenges in Evolutionary Biology. Understanding the interplay between genotype, phenotype and environment is necessary to make accurate predictions of imp ortant biological outcomes such as stress re sistance or yield in economically important plants and animals, and disease in humans. Based on population frequency patterns and footprints of selection at the DNA level, the transposable element Bari - Jheh , inserted in the intergenic region of Juvenile Ho rmone Epoxy Hydrolase ( Jheh ) genes, was previously identified as putatively ad aptive. However, the adaptive effect of this mutation remained elusive. In this wo rk, we integrate information on transcription factor binding sites, available ChIP - Seq data , gene expression analyses, and phenotypic assays to identify the functional and the mechanistic underpinnings of Bari - Jheh . We show that Bari - Jheh adds extra antioxidant response elements upstream of Jheh1 and Jheh2 genes. Antioxidant response elements ha ve been repeatedly shown to be necessary and sufficient to up - regulate downstream genes involved in oxidative stress response. Accordingly, we find that Bari - Jheh is associated with up - regulation of Jheh1 and Jheh2 and with resistance to oxidative stress i nduced by two different compounds relevant for natural D. melanogaster populations. We also show that Bari - Jheh is a dominant mutation. Further analysis with mutant flies further confirm that Jheh2 is involved in oxidative stress response. Moreover we show that TEs other than Bari - Jheh might be playing a role in the D. melanogaster response to oxidative stress. Overall our results contribute to the understanding of resistance to oxidative stress in natural populations and highlight the role of transposable elements in environmental adaptation. The replicability of fitness effects on different genetic backgrounds also suggests that epistatic interactions do not seem to dominate the genetic architecture of oxidative stress resistance.
DescriptionTrabajo presentado en "Systems Genetics and Evolution of non-human (model) organisms" (SGE2014), celebrado en Ascona (Suiza) del 18 al 22 de mayo de 2014.
URIhttp://hdl.handle.net/10261/155722
Appears in Collections:(IBE) Comunicaciones congresos
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