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In Vitro and in Vivo Study of Poly(Lactic–co–Glycolic) (PLGA) Membranes Treated with Oxygen Plasma and Coated with Nanostructured Hydroxyapatite Ultrathin Films for Guided Bone Regeneration Processes

AuthorsTorres-Lagares, Daniel; Castellanos-Cosano, Lizett; Serrera-Figallo, María Ángeles; García-García, Francisco J. ; López-Santos, Carmen ; Barranco, Ángel ; Rodríguez Gonzalez-Elipe, Agustín; Rivera-Jiménez, Cristóbal; Gutiérrez-Pérez, José-Luis
Issue Date2-Sep-2017
PublisherMultidisciplinary Digital Publishing Institute
CitationPolymers 9 (9): 410 (2017)
AbstractThe novelty of this study is the addition of an ultrathin layer of nanostructured hydroxyapatite (HA) on oxygen plasma modified poly(lactic–<i>co</i>–glycolic) (PLGA) membranes (PO<sub>2</sub>) in order to evaluate the efficiency of this novel material in bone regeneration. Methods: Two groups of regenerative membranes were prepared: PLGA (control) and PLGA/PO<sub>2</sub>/HA (experimental). These membranes were subjected to cell cultures and then used to cover bone defects prepared on the skulls of eight experimental rabbits. Results: Cell morphology and adhesion of the osteoblasts to the membranes showed that the osteoblasts bound to PLGA were smaller and with a lower number of adhered cells than the osteoblasts bound to the PLGA/PO<sub>2</sub>/HA membrane (<i>p</i> < 0.05). The PLGA/PO<sub>2</sub>/HA membrane had a higher percentage of viable cells bound than the control membrane (<i>p</i> < 0.05). Both micro-CT and histological evaluation confirmed that PLGA/PO<sub>2</sub>/HA membranes enhance bone regeneration. A statistically significant difference in the percentage of osteoid area in relation to the total area between both groups was found. Conclusions: The incorporation of nanometric layers of nanostructured HA into PLGA membranes modified with PO<sub>2</sub> might be considered for the regeneration of bone defects. PLGA/PO<sub>2</sub>/HA membranes promote higher osteosynthetic activity, new bone formation, and mineralisation than the PLGA control group.
Publisher version (URL)https://doi.org/10.3390/polym9090410
Identifiersdoi: 10.3390/polym9090410
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