Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/155233
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | A combinatorial strategy of alternative promoter use during differentiation of a heterocystous cyanobacterium |
Autor: | Muro-Pastor, Alicia M. CSIC ORCID ; Brenes-Álvarez, Manuel; Vioque, Agustín CSIC ORCID | Fecha de publicación: | 2017 | Editor: | Wiley-Blackwell | Citación: | Environmental Microbiology Reports, 9(4): 449-458 (2017) | Resumen: | Heterocystous cyanobacteria such as Nostoc sp. are filamentous photosynthetic organisms that, in response to nitrogen deficiency, undergo a differentiation process transforming certain, semi-regularly spaced cells into heterocysts, devoted to nitrogen fixation. During transition to a nitrogen-fixing regime, growth of most vegetative cells in the filament is temporarily arrested due to nutritional deprivation, but developing heterocysts require intense transcriptional activity. Therefore, the coexistence of arrested vegetative cells and actively developing prospective heterocysts relies on the simultaneous operation of somewhat opposite transcriptional programs. We have identified genes with multiple nitrogen-responsive transcriptional starts appearing in seemingly paradoxical combinations. For instance, sigA, encoding the RNA polymerase housekeeping sigma factor, is transcribed from one major nitrogen stress-repressed promoter and from a second, nitrogen stress-induced promoter. Here, we show that both promoters are expressed with complementary temporal dynamics. Using a gfp reporter we also show that transcription from the inducible promoter takes place exclusively in differentiating heterocysts and is already detected before any morphological or fluorescence signature of differentiation is observed. Tandem promoters with opposite dynamics could operate a compensatory mechanism in which repression of transcription from the major promoter operative in vegetative cells is offset by transcription from a new promoter only in developing heterocyst. | Versión del editor: | http://dx.doi.org/10.1111/1758-2229.12555 | URI: | http://hdl.handle.net/10261/155233 | DOI: | 10.1111/1758-2229.12555 |
Aparece en las colecciones: | (IBVF) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Muro-Pastor et al. 2017_TEXTO (2).pdf | 288,38 kB | Adobe PDF | Visualizar/Abrir | |
Muro-Pastor et al 2017_FIG 1-4.pdf | 749,22 kB | Adobe PDF | Visualizar/Abrir | |
FIGURE S1 Muro.pdf | 328,16 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
14
checked on 20-abr-2024
WEB OF SCIENCETM
Citations
14
checked on 25-feb-2024
Page view(s)
301
checked on 23-abr-2024
Download(s)
440
checked on 23-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.