English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/155224
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


A practical guide to build de-novo assemblies for single tissues of non- model organisms: the example of a Neotropical frog

AuthorsMontero-Mendieta, Santiago; Grabherr, Manfred; Lantz, Henrik; De la Riva, Ignacio ; Leonard, Jennifer A. ; Webster, Matthew T.; Vilà, Carles
KeywordsGenomics, Transcriptomics
Frog transcriptome
Gene ontology
Protein domain identification
Clusters of Orthologous Groups
Kyoto encyclopedia of genes and genomes
Issue Date2017
CitationPeerJ, 5:e3702 (2017)
AbstractWhole genome sequencing (WGS) is a very valuable resource to understand the evolutionary history of poorly known species. However, in organisms with large genomes, as most amphibians, WGS is still excessively challenging and transcriptome sequencing (RNA-seq) represents a cost-effective tool to explore genome-wide variability. Nonmodel organisms do not usually have a reference genome and the transcriptome must be assembled de-novo.Weused RNA-seq to obtain the transcriptomic profile for Oreobates cruralis, a poorly known South American direct-developing frog. In total, 550,871 transcripts were assembled, corresponding to 422,999 putative genes. Of those, we identified 23,500, 37,349, 38,120 and 45,885 genes present in the Pfam, EggNOG, KEGG and GO databases, respectively. Interestingly, our results suggested that genes related to immune system and defense mechanisms are abundant in the transcriptome of O. cruralis. We also present a pipeline to assist with pre-processing, assembling, evaluating and functionally annotating a de-novo transcriptome from RNA-seq data of non-model organisms. Our pipeline guides the inexperienced user in an intuitive way through all the necessary steps to build de-novo transcriptome assemblies using readily available software and is freely available at: https://github.com/biomendi/TRANSCRIPTOMEASSEMBLY- PIPELINE/wiki
Publisher version (URL)htpp://dx.doi.org/10.7717/peerj.3702
Appears in Collections:(MNCN) Artículos
(EBD) Artículos
Files in This Item:
File Description SizeFormat 
peerj-3702.pdf1,89 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.