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In vitro and in vivo assessment of digestibility of dietary oligosaccharides

AuthorsMarín-Manzano, M. Carmen; Hernández-Hernández, Oswaldo ; Rubio, Luis A. ; Moreno, F. Javier ; Sanz, M. Luz ; Clemente, Alfonso
Issue Date2015
Citation4th ICFD (2015)
AbstractPrebiotic oligosaccharides are defined as non-digestible functional ingredients which are selectively fermented and allow specific changes, both in the composition and/or activity of the gastrointestinal microbiota that confers benefits upon host well-being and health. This work summarizes the scientific evidence, based on in vitro studies, animal models and well-controlled human intervention trials, of dietary oligosaccharides in order to determine their resistance to digestion. The use of in vitro conditions, either by using digestive enzymes or homogenates of intestinal mucosa from several mammalian sources, that simulate the upper gastrointestinal tract environment has been largely employed. However, there have been only few studies to identify and quantify the intestinal survival of dietary oligosaccharides in vivo. Indeed, the elevated complexity of oligosaccharide mixtures poses a challenge in analytical chemistry that increase when biological samples are evaluated. Such complexity derives not only of the high number of compounds of the mixture but also from the different degree of polymerization and linkage type of dietary oligosaccharides. To determine the digestibility of dietary oligosaccharides in vivo, their recovery rates in feces after oral intake given to germ-free rats or to rats pretreated with an antibiotic to suppress the intestinal microbiota are considered. When animals are used, indigestible markers can be added to diets in order to quantify the digestibility rates of oligosaccharides. The use of indigestible markers allows not only evaluating the ileal digestibility of oligosaccharides but also if they are readily fermented within the large intestine by analyzing the fecal samples. In humans, the ileostomy model is considered to be a reliable and valid model to evaluate resistance to digestion, transit time and small bowel absorption of dietary compounds; nevertheless, not many candidate prebiotics has been tested in humans as yet.
DescriptionResumen del trabajo presentado a la 4th International Conference of Food Digestion, celebrada en Napoles (Italia) del 17 al 19 de marzo de 2015.
Appears in Collections:(CIAL) Comunicaciones congresos
(IQOG) Comunicaciones congresos
(EEZ) Comunicaciones congresos
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