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Frq2 from Drosophila melanogaster: Cloning, expression, purification, crystallization and preliminary X-ray analysis

AuthorsBaños-Mateos, S.; Chaves Sanjuán, Antonio; Mansilla, A.; Ferrús, Alberto ; Sánchez-Barrena, María José
KeywordsDrosophila melanogaster Frq2 Calcium-binding proteins
Issue Date2014
CitationActa Crystallographica Section F:Structural Biology Communications 70: 530- 534 (2014)
AbstractDrosophila melanogaster contains two calcium-binding proteins, Frq1 and Frq2, in the nervous system that control the number of synapses and the probability of release. To understand the differential function of the two proteins, whose sequence is only 5% dissimilar, the crystal structures of Frq1 and Frq2 are needed. Here, the cloning, expression, purification, crystallization and preliminary crystallographic analysis of Frq2 are presented. The full-length protein was purified using a two-step chromatographic procedure. Two different diffracting crystal forms were obtained using a progressive streak-seeding method and detergents. © 2014 International Union of Crystallography All rights reserved.
Identifiersdoi: 10.1107/S2053230X14005408
issn: 2053-230X
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