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http://hdl.handle.net/10261/154970
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dc.contributor.author | Moreno, F. Javier | - |
dc.contributor.author | Recio, Isidra | - |
dc.contributor.author | Olano, Agustín | - |
dc.contributor.author | López-Fandiño, Rosina | - |
dc.date.accessioned | 2017-09-11T11:52:10Z | - |
dc.date.available | 2017-09-11T11:52:10Z | - |
dc.date.issued | 2001 | - |
dc.identifier | doi: 10.1017/S002202990100471X | - |
dc.identifier | issn: 0022-0299 | - |
dc.identifier | e-issn: 1469-7629 | - |
dc.identifier.citation | Journal of Dairy Research 68(2): 197-208 (2001) | - |
dc.identifier.uri | http://hdl.handle.net/10261/154970 | - |
dc.description.abstract | The heterogeneity of caprine caseinmacropeptide (CMP) was determined by means of treatments with neuraminidase and acid phosphatase and analyses by anion exchange FPLC and reversed-phase (RP)-HPLC, with on-line and off-line electrospray ionization mass spectrometry. The main CMP components were two non-glycosylated and di-phosphorylated forms, as well as two other monophosphorylated species, each corresponding to a genetic variant of caprine κ-casein due to the silent substitution Ile/Val at position 119. Asialo-aglyco mono- and diphosphorylated forms were found in the ratios 8-14% and 86-92%, respectively. Approximately 36% of caprine CMP was glycosylated. Based on the obtained molecular masses, the occurrence of tri-, di- and monosaccharide-containing diphosphorylated CMP are reported, assuming that N-acetylgalactosamine, galactose, N-acetyl and N-glycolylneuraminic acids would constitute the main monosaccharides of caprine CMP. CMP microheterogeneity due to the genetic polymorphism was also observed in the glycosylated forms. | - |
dc.description.sponsorship | This work received financial support from the project ALI98-0969-C02-02. | - |
dc.publisher | Cambridge University Press | - |
dc.rights | closedAccess | - |
dc.subject | Caprine caseinmacropeptide | - |
dc.subject | Phosphorylation | - |
dc.subject | Genetic polymorphism | - |
dc.subject | Mass spectrometry | - |
dc.subject | Glycosylation | - |
dc.title | Heterogeneity of caprine κ-casein macropeptide | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1017/S002202990100471X | - |
dc.date.updated | 2017-09-11T11:52:10Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004837 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
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