Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/154946
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Sánchez-Blázquez, Pilar | - |
dc.contributor.author | Rodríguez-Díaz, Marta | - |
dc.contributor.author | López-Fando, Almudena | - |
dc.contributor.author | Rodríguez-Muñoz, M. | - |
dc.contributor.author | Garzón, Javier | - |
dc.date.accessioned | 2017-09-11T09:27:08Z | - |
dc.date.available | 2017-09-11T09:27:08Z | - |
dc.date.issued | 2003 | - |
dc.identifier | doi: 10.1016/S0028-3908(03)00149-7 | - |
dc.identifier | issn: 0028-3908 | - |
dc.identifier.citation | Neuropharmacology 45: 82- 95 (2003) | - |
dc.identifier.uri | http://hdl.handle.net/10261/154946 | - |
dc.description.abstract | The Gβ5 protein, which is similar in sequence to other G-protein beta subunits, mainly associates with the G-protein γ-like (GGL) domains of the R7 subfamily of regulators of G-protein signalling (RGS) proteins. This paper reports the presence of the Gβ5 protein and its mRNA in all areas of mouse CNS, and also its involvement in the cellular signals initiated at mu- and delta-opioid receptors. The expression of Gβ5 and RGS9-2 proteins (member of the R7 subfamily of RGS) was reduced by blocking their mRNAs with antisense oligodeoxynucleotides (ODN). Knock-down of these proteins enhanced the potency and duration of antinociception promoted by morphine and [D-Ala2, N-MePhe4,Gly-ol5]-enkephalin (DAMGO), agonists at mu opioid receptors. However, the activity of the selective agonist at delta opioid receptors, [D-Pen2,5]-encephalin (DPDPE), appeared to be reduced. A single intracerebroventricular (icv) ED80 analgesic dose of morphine gave rise to acute tolerance in control mice, but did not promote tolerance in Gβ5 or RGS9-2 knock-down animals. In a model of sustained morphine treatment, the impairment of Gβ5 proteins facilitated the development of tolerance. This treatment did not alter the incidence of jumping behaviour precipitated by naloxone 3 days after commencing with chronic morphine. These results show differences in the signalling regulation of G-proteins when activated by mu or delta opioid agonists. For mu opioid receptors, acute tolerance, but probably not long-term tolerance, appears to depend on the function of Gβ5 subunits and associated RGS proteins. © 2003 Elsevier Science Ltd. All rights reserved. | - |
dc.publisher | Pergamon Press | - |
dc.rights | closedAccess | - |
dc.subject | Gbeta5 proteins RGS proteins G-proteins Tachyphylaxis Acute tolerance Antisense technology Antinociception Opioid receptors Mouse brain | - |
dc.title | The GBeta5 subunit that associates with the R7 subfamily of RGS proteins regulates mu-opioid effects | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1016/S0028-3908(03)00149-7 | - |
dc.date.updated | 2017-09-11T09:27:09Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.relation.csic | Sí | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
Aparece en las colecciones: | (IC) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
37
checked on 11-abr-2024
WEB OF SCIENCETM
Citations
39
checked on 21-feb-2024
Page view(s)
216
checked on 18-abr-2024
Download(s)
84
checked on 18-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.