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Toxin production, growth kinetics and molecular characterization of Ostreopsis cf. ovata isolated from Todos os Santos Bay, tropical southwestern Atlantic

AutorMendes, Maria Cristina de Q.; Nunes, José Marcos C.; Menezes, Mariângela; Fraga, Santiago; Rodríguez, Francisco; Vázquez, José Antonio ; Blanco, Juan; Franco, José M. ; Riobó, Pilar
Palabras claveGrowth
N
P
Ovatoxins
Hemolytic assay
Phylogenetic analysis
Fecha de publicación2017
EditorElsevier
CitaciónToxicon 138: 18-30 (2017)
ResumenThe toxin profile and hemolytic activity of a strain of Ostreopsis cf. ovata (UFBA013) isolated from Todos os Santos Bay (northeastern Brazil) were evaluated under different levels of N and P. Phylogenetic analyses based on ITS rDNA region (ITS1-5.8S-ITS2) placed UFBA013 within the Atlantic/Mediterranean/Pacific clade of O. cf. ovata. Growth experiments were conducted in f/2 medium modified by adding N and P (P: 0–36 μM; N: 0–882 μM). The growth kinetics was adequately described by logistic equations. The best growth (highest Gm) was recorded under levels of N/P = 0/18, 129/5 and 441/36, while one of the lowest Gm was obtained under P-depletion. The maximum and specific maximum growth rates (as vm; cells mL−1 d−1 and μm; d−1) were achieved with N limitation (N/P = 441/36) and P-limitation/depletion (753/5.3 and 441/0) and are the highest values reported in the literature, most similar to isolates from Pacific and Mediterranean areas. The control experiment (N/P = 441/18) also yielded similar values to those from some Mediterranean isolates, but higher than formerly reported for Brazilian isolates. In all conditions assayed, no palytoxin (PLTX) was detected. The ovatoxins (OVTXs) a, b, c, d and e did not show significant differences in cell quota between exponential and stationary phases. A significant relationship was detected between OVTXs concentration and hemolytic activity
Descripción13 páginas, 6 figuras, 7 tablas
Versión del editorhttps://doi.org/10.1016/j.toxicon.2017.08.007
URIhttp://hdl.handle.net/10261/154733
DOI10.1016/j.toxicon.2017.08.007
ISSN0041-0101
E-ISSN1879-3150
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