English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/154425
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Protein kinase D1/2 is involved in the maturation of multivesicular bodies and secretion of exosomes in T and B lymphocytes

AuthorsMazzeo, C. ; Calvo, Victor ; Alonso, Roberto ; Mérida, Isabel ; Izquierdo, Manuel
Issue Date2015
PublisherNature Publishing Group
CitationCell Death and Differentiation 23(1): 99-109 (2015)
AbstractMultivesicular bodies (MVBs) are endocytic compartments that enclose intraluminal vesicles (ILVs) formed by inward budding from the limiting membrane of endosomes. In T lymphocytes, these ILV contain Fas ligand (FasL) and are secreted as 'lethal exosomes' following activation-induced fusion of the MVB with the plasma membrane. Diacylglycerol (DAG) and diacylglycerol kinase α (DGKα) regulate MVB maturation and polarized traffic, as well as subsequent secretion of pro-apoptotic exosomes, but the molecular basis underlying these phenomena remains unclear. Here we identify protein kinase D (PKD) family members as DAG effectors involved in MVB genesis and secretion. We show that the inducible secretion of exosomes is enhanced when a constitutively active PKD1 mutant is expressed in T lymphocytes, whereas exosome secretion is impaired in PKD2-deficient mouse T lymphoblasts and in PKD1/3-null B cells. Analysis of PKD2-deficient T lymphoblasts showed the presence of large, immature MVB-like vesicles and demonstrated defects in cytotoxic activity and in activation-induced cell death. Using pharmacological and genetic tools, we show that DGKα regulates PKD1/2 subcellular localization and activation. Our studies demonstrate that PKD1/2 is a key regulator of MVB maturation and exosome secretion, and constitutes a mediator of the DGKα effect on MVB secretory traffic.
DescriptionSupplementary Video 1: CMAC-labeled Raji cells (blue) were attached to fibronectin-coated MatTek chamber slides and SEE-pulsed (30 min). Double synapse formation by one GFP-CD63-expressing Jurkat cell was time-lapse imaged (right side). The video shows MVB traffic to the synapse contact areas in this cell and not in Jurkat cells that do not form synapses (left). One representative example is shown of 11 synapses recorded.
Supplementary Video 7: Synapse formed by a DsRed2-PKD1-expressing Jurkat T lymphocyte (red) with a SEE-loaded (1 μg/ml) , CMAC-labeled Raji B lymphocyte (blue). Both fluorescence channels were captured simultaneously in the video. The red channel was deconvoluted using Huygens Essential Software. DsRed2-PKD1 accumulation was observed at the synaptic contact after 30 min.
Supplementary Video 8. WT mouse T lymphoblasts were challenged with SEB-pulsed (1 μg/ml), CMAC-labeled EL-4 cells (blue) at a 1:1 ratio and transmittance and blue channels were acquired. EL-4 cells were large and blue; T lymphoblasts were smaller and irregular. White arrows indicate synaptic contacts, red arrows indicate cells that show apoptotic blebbing. T, apoptosis of effector T lymphoblasts (AICD); EL4, death of EL-4 target cells (CTL).
Publisher version (URL)https://doi.org/10.1038/cdd.2015.72
Identifiersdoi: 10.1038/cdd.2015.72
issn: 1350-9047
e-issn: 1476-5403
Appears in Collections:(IIBM) Artículos
(CNB) Artículos

Files in This Item:
File Description SizeFormat 
PKd1-2.pdf1,13 MBAdobe PDFThumbnail
Supp_1video1CDD.mov891,31 kBVideo QuicktimeView/Open
SUPP_7video7CDD.avi192,03 kBUnknownView/Open
SUPP_8video8CDD.avi3,71 MBUnknownView/Open
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.