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The Role of FBti0019985 Transposable Element in Drosophila melanogaster Adaptation: molecular mechanism and functional consequences

AuthorsMerenciano, Miriam; Ullastres, Ana ; González Pérez, Josefa
Issue Date23-Jun-2015
CitationXV Jornada de Biología Evolutiva (2015)
AbstractA way to study environmental adaptation is by elucidating the relationship between mutations and their phenotypic effect. Transposable elements are mobile DNA sequences that can induce genetic variations and have been previously shown to be a considerable source of adaptive mutations in Drosophila melanogaster. FBti0019985 is a putatively adaptive transposable element insertion present at higher frequency in out-of-Africa populations compare to Africa populations, where D. melanogaster was originated. According to the annotated reference genome, FBti0019985 has 54 nucleotides inside the 5’UTR region of CG18446 gene, which function is still unknown. We have confirmed by 5’RACE that flies with the element have the transcription start site of CG18446 inside the transposable element. Moreover these flies are more resistant to cold stress conditions than flies without the insertion. In addition, they also show higher expression levels of CG18446 gene. Recently, we have found that FBti0019985 has, at least, five different insertion sites at lower frequency around CG18446 among natural populations. We have demonstrated that FBti0019985 play an important role in D. melanogaster cold stress adaptation only when is located in the same position as it reported in the reference genome, but not when it is located in alternative sites. In other FBti0019985 alternative positions, the transposable element is inverted or too far from CG18446, preventing it from causing any phenotypic effect. We have also seen different cold stress phenotypes in different fly strains that have the insertion located in the same alternative position. Overall, these results suggest that the phenotypic effect of FBti0019985 depends on its position and also on the genetic background of each fly strain. These findings provide another example of a transposable element insertion involved in environmental adaptation.
DescriptionTrabajo presentado en la XV Jornada de Biología Evolutiva, celebrada en Barcelona el 23 de junio de 2015.
Appears in Collections:(IBE) Comunicaciones congresos
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