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Does IGF-1 have a role in the regulation of autophagy in the vertebrate inner ear?

AuthorsPulido, Sara; Rodríguez Aburto, María ; Iriarte, Rocío de ; Rodriguez-de la Rosa, Lourdes ; Magariños, Marta ; Varela-Nieto, Isabel
Issue Date2016
Citation8th GRS and IGF (2016)
Abstract[Background and aim]: IGF1 pathway regulates otic development and cochlear postnatal maturation and function. Deficiencies in IGF1 pathway have been implicated in rare and severe human syndromes that include deafness. Autophagy is a catabolic process essential for development and adult homeostasis. Autophagy provides energy for the clearing of dying cells and neuronal differentiation during early otic development. IGF1 pathway has been described to regulate autophagy in different contexts although its role is not well defined yet. Here, we will discuss the role of IGF1 in the regulation of autophagy during the early development of the otocysts (Gallus gallus) and its lifelong function in the inner ear (Mus musculus). [Methods]: The experiments were performed in ex vivo cultures of chicken otocysts treated with autophagy inhibitors and IGF1, and in wild type and Igf1 null mice. Autophagy machinery was assessed by RTqPCR and autophagy flux by Western blotting. Autophagosomes were evaluated by immunohistochemistry. [Results]: IGF1 reduced the effects of autophagy inhibitors in chicken otocysts cultures. Autophagy machinery levels were upregulated with age in the mouse inner ear. Autophagic flux increased with age. Autophagosomes were localized in spiral ganglion neurons. Although specific stages of Igf1 deficient inner ear showed a significantly different autophagic profile pattern, no definitive association with IGF1 deficiency was observed in the adult mouse inner ear. [Conclusions]: Taken together, these results show that IGF1 might play a role during early development and adult activity of the inner ear. IGF1 effects were targeted to cell survival and therefore might be indirect on the modulation of autophagy.
DescriptionResumen del trabajo presentado al 8th International Congress of the GRS and IGF Society, celebrado en Tel Aviv (Israel) del 6 al 9 de noviembre de 2016.
Appears in Collections:(IIBM) Comunicaciones congresos
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