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Attainment of critical weight is necessary for E93 up-regulation in Tribolium castaneum

AuthorsChafino, Silvia; Ureña, Enric ; Casacuberta, Elena ; Martín Casacuberta, David A. ; Franch-Marro, Xavier
Issue DateJul-2015
Citation2nd International Insect Hormone Workshop (2015)
AbstractInsects have undergone extensive evolution in their development since their origination from arthropod ancestors, such that three types of metamorphosis have emerged: ametaboly, hemimetaboly and holometaboly. These three forms of metamorphosis represent an evolving sequence from the primitive ametabolous (direct-developing) to hemimetabolous (incomplete metamorphosis) to the most derived holometabolous type of metamorphosis (complete metamorphosis). Two events are critical to promote complete metamor phosis during the onset of the last larval instar in holometabolous species: (i) the up-regulation of E93, which induces adult differentiation, and (ii) the disappearance of JH and the anti-metamorphic factor Kr-h1. Despite the importance of both events, however, the identity of the stage-specific signal(s) that control such events is poorly understood. One of the principal candidates to exert this function is the Critical Weight (CW). In holometabolous insects, the CW has been defined as the minimal size threshold that is necessary to end larval development and initiate metamorphosis. It has been reported that once critical weight is reached, JH titer declines, thus, down-regulating its target gene Kr-h1 to allow metamorphosis. Interestingly, we have also observed that CW attainment also correlates with the up-regulation of E93, suggesting a functional link between E93 and CW. To address this crucial question, we have used the beetle Tribolium castaneum as a model as this insect presents a variable number of larval stages. Under our rearing conditions, pupation takes place after seven larval instars (L1-L7). However, when we rear L7 larvae under poor-nutritional conditions, these larvae undergo new larval molting (until reaching L10) instead of pupating. Interestingly, under these conditions expression of E93 is not activated and Kr-h1 expression remains high. Remarkably, depletion of E93 in the last larval stage L7 Tribolium induces molting into new larvae. Therefore, our results show a strong relationship between the CW and the up-regulation of E93 to allow metamorphosis.
DescriptionTrabajo presentado en el 2nd International Insect Hormone Workshop, celebrado en Creta del 12 al 18 de julio de 2015.
Appears in Collections:(IBE) Comunicaciones congresos
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