English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/153802
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Astrocytic shape and glial fibrillary acidic protein immunoreactivity are modified by estradiol in primary rat hypothalamic cultures

AuthorsGarcía-Segura, Luis M. ; Torres Alemán, Ignacio ; Naftolin, F.
Issue Date1989
CitationDevelopmental Brain Research 47: 298- 302 (1989)
AbstractPrimary cultures from fetal rat hypothalamus (embryonic day 15-16) were grown for 9 days in a serum-free medium and then fixed and immunostained for glial fibrillary acidic protein (GFAP). The majority of the GFAP-immunoreactive astrocytes were flat, polygonal, without processes and showed a low intensity immunoreactivity which was restricted to the perinuclear region. Elongated, process-bearing astrocytes, with an intense immunoreactivity in the soma and processes, were also observed in a smaller proportion (30%). Addition of estradiol (10-12 to 10-8 M) to the culture medium 24 h before GFAP immunostaining resulted in an increased proportion (59-69%) of process-bearing, intense immunoreactive cells. This effect was blocked by tamoxifen (10-8 M). The total number of GFAP-immunoreactive astrocytes was not modified by estrogen or tamoxifen. These results indicate that estradiol may modulate the cell shape and the distribution of GFAP in astrocytes in culture and suggest that astrocytes can be a target for sex steroids during development of the central nervous system. © 1989.
URIhttp://hdl.handle.net/10261/153802
DOI10.1016/0165-3806(89)90186-7
Identifiersdoi: 10.1016/0165-3806(89)90186-7
issn: 0165-3806
Appears in Collections:(IC) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.