English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/153752
Título

The abundance of Fob1 modulates the efficiency of rRFBs to stall replication forks

AutorCastán, Alicia; Hernandez, Pablo ; Krimer, Dora B. ; Schvartzman, Jorge Bernardo
Palabras claveDNA
Ribosomal electrophoresis
Agar gel
Eukaryotic cell
Gel
Genes
Ribosomes
Saccharomyces cerevisiae
Saccharomycetales
Tandem repeat sequences
Clinically isolated syndrome
Spacer device
DNA replication fork
Binding (molecular function)
Fecha de publicación26-jul-2017
EditorOxford University Press
CitaciónNucleic Acids Res gkx655 (2017)
ResumenIn eukaryotes, ribosomal genes (rDNA) are organized in tandem repeats localized in one or a few clusters. Each repeat encompasses a transcription unit and a non-transcribed spacer. Replication forks moving in the direction opposite to transcription are blocked at specific sites called replication fork barriers (rRFBs) in the non-transcribed spacer close to the 3΄ end of the transcription unit. Here, we investigated and quantified the efficiency of rRFBs in Saccharomyces cerevisiae and to this end transfected budding yeast cells that express dissimilar quantities of Fob1 with circular minichromosomes containing different copies of the minimal 20-bp DNA segment that bind Fob1. To identify fork stalling we used high-resolution 2D agarose gel electrophoresis. The results obtained indicated that neighbor DNA sequences and the relative abundance of Fob1 modulate the efficiency of rRFBs to stall replication forks.
Descripción14 p.-7 fig.
Versión del editorhttps://doi.org/10.1093/nar/gkx655
URIhttp://hdl.handle.net/10261/153752
DOI10.1093/nar/gkx655
ISSN0305-1048
E-ISSN1362-4962
Aparece en las colecciones: (CIB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
NAR 2017.pdfArtículo principal6,2 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.